Growth medium: You are running different experiments on microbes and need to make sure you choose the correct medium.

Human Anatomy & Physiology (11th Edition)
11th Edition
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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**Growth Medium Selection Guide:**

This guide helps in selecting the appropriate growth medium for various microbial experiments. Below are the requirements and corresponding media options:

1. **Need to grow a wide range of bacteria and fungi from the environment:**
   - **Options:** Choose from available media.

2. **Need to select for the growth of fungi (yeasts & molds):**
   - **Options:** 
     - Sabouraud-Dextrose Agar (SDA)

3. **Need to grow fastidious bacterial pathogens of humans:**
   - **Options:** 
     - SCC Agar (SCCA)

4. **Need to differentiate bacteria based on hemolysis:**
   - **Options:** 
     - Blood Agar (BA)

5. **Need to select for salt tolerant bacteria:**
   - **Options:** 
     - MSA

6. **Need to select for members of genus _Staphylococcus_ in pus from an abscess:**
   - **Options:** Choose from available media.

7. **Need to select for members of bacterial family Enterobacteriaceae, e.g., E. coli, Enterobacter, Salmonella, Yersinia, Klebsiella, Proteus:**
   - **Options:** 
     - MacConkey Agar (Mac)

8. **Need to differentiate between lactose fermenting and non-lactose fermenting enterics:**
   - **Options:** 
     - EMB

**Available Media Options:**
- Sabouraud-Dextrose Agar (SDA)
- MacConkey Agar (Mac)
- SCC Agar (SCCA)
- MSA
- Tryptic Soy (TS broth or TSA)
- O'Dougal's Agar (ODA)
- Blood Agar (BA)
- Sacto Agar (SA)
- EMB

This guide assists in optimizing your experimental conditions by choosing the correct growth medium based on the specific microbial requirements.
Transcribed Image Text:**Growth Medium Selection Guide:** This guide helps in selecting the appropriate growth medium for various microbial experiments. Below are the requirements and corresponding media options: 1. **Need to grow a wide range of bacteria and fungi from the environment:** - **Options:** Choose from available media. 2. **Need to select for the growth of fungi (yeasts & molds):** - **Options:** - Sabouraud-Dextrose Agar (SDA) 3. **Need to grow fastidious bacterial pathogens of humans:** - **Options:** - SCC Agar (SCCA) 4. **Need to differentiate bacteria based on hemolysis:** - **Options:** - Blood Agar (BA) 5. **Need to select for salt tolerant bacteria:** - **Options:** - MSA 6. **Need to select for members of genus _Staphylococcus_ in pus from an abscess:** - **Options:** Choose from available media. 7. **Need to select for members of bacterial family Enterobacteriaceae, e.g., E. coli, Enterobacter, Salmonella, Yersinia, Klebsiella, Proteus:** - **Options:** - MacConkey Agar (Mac) 8. **Need to differentiate between lactose fermenting and non-lactose fermenting enterics:** - **Options:** - EMB **Available Media Options:** - Sabouraud-Dextrose Agar (SDA) - MacConkey Agar (Mac) - SCC Agar (SCCA) - MSA - Tryptic Soy (TS broth or TSA) - O'Dougal's Agar (ODA) - Blood Agar (BA) - Sacto Agar (SA) - EMB This guide assists in optimizing your experimental conditions by choosing the correct growth medium based on the specific microbial requirements.
## Microbial Differentiation Techniques

### Differentiation between Lactose Fermenting and Non-Lactose Fermenting Enterics
- **Objective**: To identify lactose fermenters and non-fermenters among enteric bacteria.
- **Tool**: [Choose]

### Selection and Differentiation of _E. coli_ from Other Enterobacteriaceae
- **Objective**: To isolate and distinguish _E. coli_ from most other members of the Enterobacteriaceae family.
- **Tool**: [Choose]

### Screening for Fecal Contamination Indicators in Food and Water Samples
- **Objective**: To identify possible fecal contamination in various samples.
- **Tool**: [Choose]

### Distinguishing Mannitol Fermenting and Non-Mannitol Fermenting _Staphylococcus_
- **Objective**: To differentiate between _Staphylococcus_ species based on their ability to ferment mannitol.
- **Tool**: [Choose]
Transcribed Image Text:## Microbial Differentiation Techniques ### Differentiation between Lactose Fermenting and Non-Lactose Fermenting Enterics - **Objective**: To identify lactose fermenters and non-fermenters among enteric bacteria. - **Tool**: [Choose] ### Selection and Differentiation of _E. coli_ from Other Enterobacteriaceae - **Objective**: To isolate and distinguish _E. coli_ from most other members of the Enterobacteriaceae family. - **Tool**: [Choose] ### Screening for Fecal Contamination Indicators in Food and Water Samples - **Objective**: To identify possible fecal contamination in various samples. - **Tool**: [Choose] ### Distinguishing Mannitol Fermenting and Non-Mannitol Fermenting _Staphylococcus_ - **Objective**: To differentiate between _Staphylococcus_ species based on their ability to ferment mannitol. - **Tool**: [Choose]
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