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A culture medium consisting of known amounts of glucose, NaCl, and ammonium and potassium phosphate would be an example of:
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- The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…A pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To prepare the solution, he dissolves Lidocaine HCl in purified water to make an isotonic solution. Then he dilutes the Lidocaine HCl solution with 0.9%w/v sterile sodium chloride to complete the formulation. How many milliliters of purified water is needed to make the formulation by this method?SEE EXAMPLE IN THE IMAGE A mixture of 0.20M acetic acid and 0.30M sodium acetate is given. Calculate the pH of the medium if the pKa of the acetic acid is 4.76. Show your complete solution.
- Using the sodium chloride equivalent method, make the following solution isotonic with respect to the mucous lining of the eye (ocular membrane): Tetracycline hydrochloride: 10 g NaCl: x g Sterile distilled water, enough to make 1000 mL Report your answer in grams (to one decimal place).You have a 1000X stock solution of sodium chloride (NaCl). The concentration of NaCl in the stock solution is 5M. a)How would you make 300mL of a 1X solution? b)What is the concentration of NaCl in your 1X solution?A 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?
- Give the solution ASAP.Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?A solution contains 1.0 g of ephedrine sulfate in a volume of 100 mL. What quantity of sodium chloride must be added to make the solution isotonic? E value for the drug is 0.23.
- Give the complete solution.Given 10 gram of vitamin C (E=0.18), we can make ml of isotonic Vitamin C solution.The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.