Consider the Rho-dependent terminator sequence 5’CCCAGCCCGCCUAAUGAGCGGCCUUUUUUUU-3’. What affect would a point mutation at any one of the bolded and underlined nucleotides disrupt termination of transcription? Group of answer choices 1.Mutation in one of these nucleotides would disrupt base pairing, but not affect the formation of the hairpin and termination proceeds. 2.Mutation in one of these nucleotides would have no affect on base pairing, so the termination hairpin is formed and termination proceeds. 3.Mutation in one of these nucleotides would not disrupt base pairing, but would prevent the formation of the hairpin and disrupt termination. 4.Mutation in one of these nucleotides would disrupt base pairing, preventing the formation of the hairpin and disrupting termination.

Identify the DNA elements and protein factors, #1-7, in the figure below that are involved in the initiation and elongation of transcription at a eukaryotic promoter of a gene TRANSCRIPTION ON RNA PROCESSING PefA A eukaryotic promoter commonly includes a TATA box (a nucleotide sequence TRANSLATION Ritoom containing TATA) about 25 nucleotides upstream from Nontemplate (coding) strand of DNA 7 Pupeptide 1 the transcriptional start point. Nontemplate (coding or sense) strand DNA TATAAAA ATATILTI 5 2 3 Start point 2 Several transcription TCCAA 3' 5' factors, one recognizing the TATA box, must bind 3' end to the DNA before RNA 4 polymerase Il can bind in the correct position and orientation. UCCA 3 5' 3' 3 Additional transcription GGTT factors (purple) bind to the DNA along with RNA 5' Direction of transcription polymerase II, forming the transcription initiation 3 complex. RNA polymerase Il then unwinds the DNA double helix, and RNA synthesis begins at the start point on the temple strand.…

(11) If you design a gRNA with the sequence, GGU AUC AUU GCA CUG ACC AG, in theory which position of the nucleotide in the genomic DNA sequence below will be cut by Cas9 protein, which may lead to a knockout due to an error in DNA repair? The starting and the end coordinate positions of the DNA fragment on the chromosome are indicated at the beginning and end of the sequence, respectively. 110,001 ATC GGT ATC ATT GCA CTG ACC AGA GGC TAC 110,030 A) Between 110,023 (G) and 110,024 (A) B) Between 110,021 (C) and 110,022 (A) C) Between 110,020 (C) and 110,021 (C) D) Between 110,009 (C) and 110,010 (A)

Keeping in mind the three main processing events that occur in eukaryotes (5' capping, 3' cleavage and intron splicing) would it be reasonable to conclude that the mature RNA would contains exons, a promoter region (to the left of the transcription site) and a poly A tail?