A graduate student is trying to create a more efficient in vitro transcription system. The students idea is to covalently attached the sigma factor to RNA Polymerase so that the complex will be more efficient at binding promoter. Is this a good idead?

Identify the labeled factors in the figure below (A and B) and indicate the direction of transcription. ts A O A= Mediator, B= TFIID; direction is into the screen O A= TEIIE, B= TFIIH; direction is into the screen rences A= TBP, B= TEIIB; direction is out of the screen O A= TFIIF, B= TFIIE; direction is out of the screen

How does the sigma factor of E. coli RNA polymerase bind to promoters?Question 10 options: a region of the protein binds to a stem-loop structure in the DNA a region of the protein binds to a stem-loop structure in the RNA a region of the protein inserts into the minor groove of the DNA a region of the protein binds to the negatively charged backbone of the DNA a region of the protein inserts into the major groove of the DNA

Now it is time to put all of the elements of transcription together. Write an essay below to describe the process by which mRNA is formed. Use these terms correctly in your essay, and underline each one: TATA box, gene, terminator, promoter, elongation, 5' to 3', termination, initiation RNA, polymerase RNA nucleotides, template, start point, termination signal, and transcription factors.