What is the regulatory region of the Trp operon that is involved in alternative transcription termination mechanism? Choose from the options below. Attenuator  Promoter   Leader sequence Repressor  Operator

What is a specific region in the trp operon that you could mutate so it expresses the trp operon in conditions of excess tryptophan. Why will this mutation cause this? Operon structure: promoter, operater, structural genes promoter -  DNA region that is the site of RNA polymerase sigma binding and transcription initiation operator – DNA region that influences gene expression through its interaction with a protein (transcription factor) structural genes – gene whose expression is being regulated; encodes a polypeptide or functional RNA

The operator of the lactose operon in E. Coli is located:     between the promoter and the repressor gene     upstream of the promoter     between the promoter and the structural genes     anywhere on the chromosome     among the structural genes

The organization and function of the lac operon in E. coli is shown in the following figures: XXI Transcription Repressor mRNA Translation RNA polymerase M M Flask ZYA XXXX Active M-repressor protein Repressor active, operon off. The repressor protein binds with the operator, preventing transcription from the operon. (a) An inducible operon Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Allolactose (inducer) Carbon/Energy Source 0 Z MInactive repressor protein (a) An inducible operon Transcription Translation XXX Permease B-Galactosidase Transacetylase Operon mRNA 3 Repressor inactive, operon on. When the inducer allolactose binds to the repressor protein, the inactivated repressor can no longer block transcription. The structural genes are transcribed, ultimately resulting in the production of the enzymes needed for lactose catabolism. Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Suppose you inoculate three flasks of minimal…