a eukaryotic protein involved in immunity, and you are attempting to express this protein in E. coli in order to produce large amounts of the protein. You take your DNA construct and place it into E. coli. You measure protein expression and notice that no protein is being made. Provide 1 reason why you don't see protein as it relates to transcription and how this problem could be resolved.

Which of the following mutations in the protein-coding region of a gene is more likely to lead to complete loss of function of the encoded protein: an insertion of six nucleotides or a deletion of two nucleotides? Briefly explain your answer.

Describe the initiation step of bacterial transcription (make sure to include important enzymes, parts, and sequences in your answer) up to the beginning of elongation. What are three ways that transcription differs between bacteria and eukaryotes? (I'm not looking for types of RNA modification, just in terms of transcription)

Using the transcription unit diagrammed below, in which exons are represented by blue boxes and introns are represented by the connecting lines.  You discover a single base deletion in region E of this DNA sequence. Regarding transcription, this mutation will likely: 1.) Result in an alteration to the mRNA sequence. 2.)Have no effect on transcription or the mRNA sequence 3.)Prevent transcription at the TATAA box 4.) Result in an increase or decrease in the amount of mRNA transcribed

Protein X is a secretory protein that is synthesized on ER-bound polyribosomes in vivo. You synthesized this protein in an in-vitro protein translation system in the absence of microsomes. You add microsomes post-translationally to this system. What do you expect to see? Group of answer choices The signal sequence of the protein will be cleaved off The protein will be translocated across the microsomal membrane. The protein will be sensitive to the action of proteases The protein will be glycosylated All of the answer choices are correct