a eukaryotic protein involved in immunity, and you are attempting to express this protein in E. coli in order to produce large amounts of the protein. You take your DNA construct and place it into E. coli. You measure protein expression and notice that no protein is being made. Provide 1 reason why you don't see protein as it relates to transcription and how this problem could be resolved.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
You are interested in a eukaryotic protein involved in immunity, and you are attempting to express this protein in E. coli in order to produce large amounts of the protein. You take your DNA construct and place it into E. coli. You measure protein expression and notice that no protein is being made. Provide 1 reason why you don't see protein as it relates to transcription and how this problem could be resolved.
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