One of Dr. Peῆa’s doctoral students, Marisol, finds out there are multiple mutant forms of U ribozymes using BLAST. Remember that U ribozymes make up part of the spliceosome . Will this news be of any benefit?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Antibiotic-resistance is one of the gravest dangers to the world’s medical system. As a result, Dr. Jhon Peῆa decides to apply for a research grant from the NIH to investigate novel targets for antibiotic treatment in his lab. Most bacteria have developed resistance to counter the effects of the current crop of antibiotics, which largely target the cell wall, plasma membrane, or ribosomes. Dr. Peῆa’s team has instead been investigating bacterial transcriptional sites and all of his students are asked to attend a group Zoom meeting to discuss ideas to put into the grant.

 

 

1) 

One of Dr. Peῆa’s doctoral students, Marisol, finds out there are multiple mutant forms of U ribozymes using BLAST. Remember that U ribozymes make up part of the spliceosome . Will this news be of any benefit?
 
a
YES! LOF in any of the U ribozymes will halt splicing, which is necessary before a transcript can be translated.
b
NO! Bacteria are capable of alternative splicing so they will use a different method to splice the transcript if need be.
c
YES! The gene for the U ribozyme can be transferred into the bacteria using the same engineering method that moved a human insulin gene into them.
d
NO! Bacteria do not have a spliceosome.
 
2)  
Suroj, Dr. Peῆa’s newest master’s student, asks if anyone has considered looking into polyadenylase, an exonuclease that destroys 3’ poly-A tails. Is this going to get the lab a grant?
 
a
NO! Bacteria do not have poly-A tails!
b
YES! Bacteria have poly-A tails to help with orientation; if the tail is removed, the bacteria may be translated in the wrong direction producing a defective protein that should kill it.
c
First one would need to find out what bacteria, if any, have poly-A tails attached to the 3’ end of the transcript, and if so, are any of them pathogenic.
d
Not sure this would be helpful since poly-A tails in bacteria cause the transcript to be degraded quicker already; if you mess it up, that means it won’t be produced and the bacterial transcripts will last longer.
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