The standard curve of given data points is plotted in excel sheet as shown below: Absorbance (AU) vs Concentration 0.28 0.3 0.25 2 0.2 0.15 0.08 0.1 0.06 0.05 0.03 3 Concentration (M) Absorbance (AU)
Q: 0.0050 M KOH PH VALUE
A: KOH stands for potassium hydroxide. It is an inorganic compound with the chemical formula of KOH.
Q: Explain how the following will affect blood film or smear a. Humidity b. Temperature c. Speed d.…
A: White blood cells (WBCs, leucocytes), red blood cells (RBCs, erythrocytes), and platelets can all be…
Q: 8 7- 6- 2- 1 405 180 225 270 315 [Substrate] (nM) 45 90 135 360 450 495 540 58! What are the units…
A: Solution Michaelis menten kinetics is one of the best models of enzyme kinetics. Value of…
Q: When calibrating a pH meter, 3 (three) standard color-coded buffer solutions are used, which of…
A: pH is a quantitative measure of how acidic or basic is the aqueous or other liquid solution is. It…
Q: Based on the ternary phase diagram, a binary mixture of furfural and water will always split into…
A: * Ternary diagram is triangular coordinate system with axes at edges these are used to plot three…
Q: relative density of the insoluble powder
A:
Q: There are several different units that biochemists use to describe the concentration of a solution.…
A: BSA means bovine serum albumin. Given Values: Mol. wt of BSA = 80000 Dalton or 80 kD Weight of the…
Q: Below is a Beer's Law Plot for the concentration of a specific colored compound x. A solution of…
A: Beer Lambert's plot It's a graph showing a compound's absorbance vs. concentration. It is a straight…
Q: You purchase a premix flour containing iron (Fe), riboflavin, niacin, thiamin, and folate. For…
A: Atomic absorption spectrometry (AAS) is a simple, high-throughput, low-cost technique for analyzing…
Q: 0.47 absorbance/ 4713.6 mol/L = In scientific notation please & 0.57 abosorbance/4713.6 mol/L=
A: Beer-Lambert law states a linear relationship between concentration and absorbance. When…
Q: What is the approximate concentration of the solution in mol\kg, if 1 mol of solute particles raises…
A: Boiling point elevation describes the phenomenon that the boiling point of a liquid will be higher…
Q: What do you call to the unit amount of a substance used for measuring large quantities of very small…
A: The mole (particularly millimoles and molarity) is a commonly used unit in medicine. For example,…
Q: A drug has a measured diffusivity of 5*10^-6 cm^2/s^-1 at 37 degree Celsius. What is this drug’s…
A: The Stokes-Einstein equation is used to measure the viscosity of various solutions, and the…
Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml)…
A: Biuret is a compound that formed when urea is warmed, which makes two urea particles to join. Urea…
Q: BSA Concentration (ug/mL) Abs 2000 2.25 1500 1.715 1000 0.915 750 1.156 500 0.714 250…
A: A standard curve is defined as a calibration curve that is used for quantitative estimations. In…
Q: How many grams of NaOH would you need to make 7,252 mL of a 230 mM NaOH solution (m.w. = 40.00…
A: Answer- Here molarity of the solution is provided and requires the amount of weight of NaoH.
Q: The main constituents in vinegar are water and ethanoic acid (CH3COOH). In order to determine the…
A: You have asked question with parts. I will be answering the first three parts. a) You should take…
Q: You want 235mL of a solution that consists of 10% BSA, 2.37mM KCl and 120µM EDTA. You have stock…
A: BSA is a globular proteins which is a well studied proteins and used as standard protein molecule in…
Q: When 0.5 mol of a certain ionic substance is dissolved in 1.0 kg of water, the freezing point of the…
A: The physical properties (freezing and boiling point) of a solvent caries n addition of solute. The…
Q: The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L =…
A: Asked : Effect on measured pH value if acid concentration is increased HCl is an acid
Q: If the equation produces from data is y = 0.300x - 0.857 and the absorbance for the unknown is…
A: Note: Since you have posted multiple independent questions in the same request, we will solve the…
Q: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530…
A: Biuret test is a chemical test used for detecting the presence of peptide bonds, in whose presence…
Q: What wavelength is most suitable for quantitative analyses for an analyte exhibiting the UV-Vis…
A: Quantitative analysis is the method of determination of concentration of an analyte where teh amount…
Q: A 2.5mg/ml solution of ampicillin is available for use, what volume would you add to 200ml of LB…
A: Ampicillin is a broad spectrum semi-synthetic derivative of penicillin. It is active against both…
Q: If you had measured the absorbances of your unknown in test tubes that were half the diameter of the…
A: Proteins are composed of twenty standard amino acids that are attached together via peptide bonds.…
Q: Use the information in the figure, below. Include units in your answer. Round numbers to 3 decimal…
A: BSA standard curve: Bovine Serum Albumin(BSA) is a Globular protein that is being used as a standard…
Q: A patient is prescribed 15mmol of potassium chloride injection. The ampoule contains 20mmol in 10ml.…
A: Given data A 10 ml ampule contains = 20 mmol
Q: For a powder binding process that mixes a 1:2 volume ratio mixture of semi-crystalline polyethylene…
A: Given: Density of semi-crystalline substance = 0.900 g/cm3 Density of crystalline substance = 0.998…
Q: In a spectrophotometer, what should be the appearance on the graph if the sample is pure? presence…
A: Spectrophometry is a widely used technique in identification and confirmation of chemical…
Q: If a liquid contains 3 mg of drug in 40 mL, what is the milligram percent of this solution? Round to…
A: Drug calculation is an essential component of drug administration. As Nurses we need to understand…
Q: Given the following data set, which cuvettes could be used to make a standard curve for absorption…
A: A standard curve is made using known quantities and it can be used to estimate unknown quantities.…
Q: If 10g of salt was mixed in 1L water. What percent solution will this be.
A: According to the question,Mass of solute = 10 grams = 10gVolume of water = 1L = 1000 mili Liters…
Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) 0 1 2 3 4 5…
A: Biuret test is a quantitative method to determine the total protein concentration in any unknown…
Q: Using the following plot, calculate the change in entropy (J/mol K). 2.000 In(K,) vs 1/T(K) 1.500…
A: In the field of chemical kinetics, Arrhenius plot gives the log of a reaction rate constant ln (k),…
Q: BSA (mg/ml) Absorbancy 595nm 0.225 1 0.310 2 0.420 3 0.510 4 0.610 0.720 6 0.810 7 0.915 8 0.950…
A: The Bradford Assay is is a sensitive assay to detect and quantify protein in solution.
Q: f there is a stock solution of 2000 (ug/ml) , is it possible to use SERIAL DILUTION to generate the…
A: The stock solutions are the concentrated solutions which can be used to prepare the solutions of…
Q: The solubilities of three salts in water are shown in the graph. 1000 Pb(NO,), 900 For each salt,…
A: 500 gm of each salt is mixed with 1 L water at 40 degree Celsius.
Q: ·A rectangular solid measures 8 cm long by 2 cm wide by 2 cm high. What is the…
A: Answer. 9/4 Explantion:- Given:- Rectangular Solid with measurement:- 8 cm long X 2 cm wide X 2…
Q: The k of water is 1.86 kg °C/mol, approximately 4x the value of k,. If you attempted to freeze your…
A: Freezing point depression is a colligative property and it is noticed in solution when solute…
Q: Assume that you have a stock solution of 10% SDS and you need to make 150 microliters of 0.5% SDS.…
A: Stock solution (SDS) = 10%Concentration of stock solution = 10g of SDS/ 100ml of water.Similarly,…
Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) 0 1 2 3 4 5…
A: Even when the concentration of BSA (Bovine Serum Albumin) is 0 , an absorbance of 0.158 was…
Q: The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L =…
A: Asked: Correct option out of the given options
Q: How does the results of the pH meter and the pH paper compare with each other?
A: pH is the concentration of hydrogen ions present in the given solution. The determination of the pH…
Q: A slice of pizza has 500 kcal. If we could burn the pizza and use all the heat to warm a 50-L…
A: Specific heat is a physical property of substance. It is the amount of heat needed per unit mass to…
Q: 10 Determine the Hill coefficient for the following binding data using a linear method. [L] in…
A: The Hill plot is a graph graph which enables us to check whether the protein in question exhibits…
Q: How many liters of a 7 M NaOH stock solution would you need to make 780 mL of a 153 mM NaOH…
A: Molarity is defined as the number of moles of solute present in a liter of solution. Molarity =…
Q: If skin cleaning solution is to be diluted 1 teaspoon to 64 ounces of water, how much water would…
A: GivenOne teaspoon of skin cleaning solution is diluted to 64 ounces of water.To findWater needed to…
Q: If you mix 9 g of NaOH with 90 mL of water, what is the mass/volume (g/mL) concentration of the…
A: Given Values: Weight of NaOH = 9 gVolume of the water to be mixed with 9 g NaOH= 90 ml
Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy…
A: The Biuret test is used to find the amount of protein in the given analyte as it checks the presence…
Q: What information do we get if we calculate the ratio of the Absorbance value at 260 nm to the…
A: A spectrophotometer is a device that is used to calculate the absorbance of light passing through a…
Q: Using the table above, make the following unit conversions. Determine th point the appropriate…
A: 1. 25/1000 = 0.025 2. 3*1000000= 3000000 3. 150/10 = 15 4. 5000/1000 = 5 5. 0.075 kl
Q: You are a graduate student who is trying to conduct an experiment. For this experiment, you need to…
A: In order to make working solutions, it is often required to dilute the available stock solutions.…
Q: If the dehydration reaction of an alcohol is successful, what changes would be seen in the IR…
A: Biochemistry is a branch of biology that deals with the study of chemical processes within living…
Now calculate the slope and equation for the best fit line. You may use excel
Trending now
This is a popular solution!
Step by step
Solved in 6 steps with 6 images
- BSA Concentration (ug/mL) Abs 2000 2.25 1500 1.715 1000 0.915 750 1.156 500 0.714 250 0.082 125 0.086 25 0.082 Create a BSA standard curve Abs562=f[BSA] using a Scatter Plot representation in Excel.You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equationThe standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
- A mixture containing only KCl and NaBr is analyzed by the Mohr Method. A 0.3172-g sample is dissolved in 50 mL water and titrated to the Ag2CrO4 endpoint, requiring 36.85 mL of 0.1120 M AgNO3. A blank titration requires 0.71 mL of titrant to reach the same endpoint. Report the % (w/w) KCl and NaBr in the sample. [Ans . 84.41 % (w /w); 17.59 % (w /w)]The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if HCl in pH electrode, became 0.15M instead of 0.1M ? a.impact can not be determined b.measured pH is higher than "real" pH. c.measured pH is lower than "real" pH. d.measured pH is same as "real" pH.i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision
- Using the table above, make the following unit conversions. Determine this by moving the decimal point the appropriate number of places. 1. 25 μL mL 8. 8500 µL = 2. 3 L = µL 9. 64 TL = GL 3. 150 cL = dL 10. 120000 nL = µL 4. 5000 pL = nL 5. 75 L = kL 6. 800 nL = cL 7. 6 hL = ML In addition to being able to convert among different units of measurement, geneticists also need toYou obtained the following raw data when setting up a Biuret standard curve: Absorbancy BSA (mg/ml) 540nm 0.158 1 0.210 0.260 0.305 0.360 0.410 0.455 7 0.510 0.530 0.550 10 0.554 What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places) O-2N 34 56 o9The standard curve provided below will allow you to answer the remaining questions. Generic Standard Curve 0.7 y = 0.9425x- 0.0034 0.6 0.5 0.4 0.3 02 0.1 0.1 0.2 0.3 0.4 0.5 0.6 0.7 Concentration (mg/ml) - Please describe two ways in which you could use this standard curve to determine the concentration of a pigment in an unknown sample.| #1: #2: -What is your rough estimation of the concentration if you determine the absorbance of a sample is 0.150? Absorbance
- You obtained the following raw data when setting up a Biuret standard curve: Absorbancy BSA (mg/ml) 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 10 0.550 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).Compute for the sodium concentration of the patient. Use the same formula in the computation of the concentration using absorbance and concentration of the standard. Given: Standard sample Concentration : 150mmol/L Absorbance: 0.112 Patient's sample Absorbance : 0.226 Concentration : ??The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pH