You treat cells briefly with radioactively labeled cysteine and then transfer them to a medium with unlabeled cysteine. Where would you expect to detect radioactivity immediately after the transfer to the unlabeled medium? Where would you expect to detect it an hour later?
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You treat cells briefly with radioactively labeled cysteine and then transfer them to a medium with unlabeled cysteine. Where would you expect to detect radioactivity immediately after the transfer to the unlabeled medium? Where would you expect to detect it an hour later?
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- As part of an experiment where absorbance values are measured using a spectrophotometer, you are taking readings of your sample every 20 minutes. The non-motile microbe you're testing has a generation time of roughly 20 minutes at an incubation temperature right around room temperature. Things start out fine, with the expected results — as time goes by at the correct incubation temperature, absorbance starts to rise as the medium starts to become more cloudy with growing microorganisms. But roughly 2 hours into the process, you notice that the absorbance levels flatten out, and then start to decrease unexpectedly. What is most likely taking place in your experiment?Your instructor asks you to isolate and identify the organisms in an unknown culture. You find that the culture contains two gramnegative bacilli that produce swarming colonies. What biochemical test would you use to identify the bacilli? Justify your answer.1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2) Which of the following is the main goal when staining a culture? A) improve contrast B) identify bacteria C) Visualize bacterial structures
- Which would move faster in thin layer chromatography?a) Beeswaxb) Phosphatidylinositolc) Cholesterold) SteroidPrior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 1 (1) (ii) 3 (iii) 2 (iv) 4 Note: Count cells on the four labelled squares. Include cells touching the line on top and left. 18P 06. ¿66 Ⓡ Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.What cell types would be able to grow on the ECM in the following situations?a) Streptomycin was not added to the ECM.b) The ECM contains thiamine.c) The ECM contains all 20 amino acids and all 5 nitrogenous nucleic acid bases. Reminder: ECM = minimal medium + glucose + has streptomycin antibiotic
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