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Please help me answer how much volume of cell suspension needed (for 5*10^4 cells) in microliters. Pertinent Information shown in the image.
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- 6.1 EXPECTED RESULT FOR CELL COUNT USING HAEMOCYTOMETER Table 2 Number of cell in upper grid 15 13 Grid location number Number of cell in lower grid 20 17 1 3 4 10 14 18 13 7 20 15 17 14 13 10 10 12 13 18 Total cells in upper and lower grid 120 120 TOTAL CELLS 240 Total grids 16 Please calculate a numbers of cell/ml from the information table 2.You have determined that a cell suspension contains 1 x 103 cells/mL. That cell suspension was prepared by adding 1 ml of a more concentrated cell suspension to 999 mL of phosphate buffered saline. What is the concentration of cells in the original cell suspension?When doing a routine differential count for a CBC how many cells are counted? a) 50 b 75 c 100 d 125 e 150
- Upon dislodging cells from the plate, you had the following cell counts using 4 different squares of the hemocytometer: (83,80,75,79) i) Please calculate the number of cells per ml. ii) Please provide your calculation to obtain 0.5X106 cells/ml in 2mls cell suspension.O minute 2 minutes 5 minutes #12 #13 Identify the cell type, (plant or animal).Please count the cells in grids 1, 3, 7, and 9. Calculate the cell density (a) and cell viability (b) for this sample. (assume we started with 100 microliters of cells and added 100 microliters of trypan viability blue). Hemacytometer Grid 123 5 6 789 4 Enlarged view O O O 8
- After blood collection, the red cells are separated from the serum to be used for the preparation of the stock solution. How is it done? For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What is the appropriate diluent used for the preparation of the red cell suspension?If a red blood cell was placed in a 50mOSM solution, the cell would:You look through a compound microscope and count 8 cells that fit across the diameter of your field of view with the 40X lens. Your field diameter is a 4X lens and is 5 mm. Answer the following questions below: (show work for full credit): What is the field diameter at 40X? What units are you using? mm or um? ( make sure to explain your answer) What is the formula for calculating cell size?
- Label the nucleus, cytoplasm, and cell membrane of the following pictures. The red one is the human red blood cell under HPO, while the orange one is the toad red blood cell. Describe each cell and compare the two.A normal red blood cell is small and shaped like a flattened disk. The interior of a red blood cell has a salt concentration around 0.9%. Suppose red blood cells were dropped into beakers of different saline solutions as shown below. In which of these solutions would the cell: rupture, shrink, or stay the same? 0.9% red blood cell 0.1% 2.9%Your supervisor gave you a 5% saline (NaCl) solution. You need to make 100ml of 1.0% saline solution. How much 5% saline would you take to complete the task?