Given 10 gram of vitamin C (E=0.18), we can make ml of isotonic Vitamin C solution.

Q: Consider a solution of glucose in water that is 5.25% by mass glucose. Calculate the indicated…

A: Water contains 5.25% by mass glucose. Mass of the solution=225g  Therefore, Concentration of…

Q: If I want to make a 100mL buffer that has 2% SDS, 100mM tris pH 8, 10mM EDTA, and proteinase K…

A: Introduction: Buffer is defined as a solution that resists change in pH on the addition of acid or…

Q: The following stock solutions are available to make a protein extraction buffer: 100% Nonidet P-40,…

A: Molar concentration, also known as molarity, it is a number of moles of solute in a solution. Molar…

Q: (MB = methylene blue) What is the concentration of the sample of the substance with the reporter…

A: Here the absorbance of the methylene blue sample is given to be: 0.40 at 595 nm. According to the…

Q: You want to make a solution that is 1x TE AND 10% glycerol in the same solution. You have the…

A: A percentage solution can be defined as the amount of volume of a particular chemical compound that…

Q: Given a stock protein solution with a concentration of 3 mg/ml, determine the protein concentration…

A: Stock solutions are used in many laboratory procedures, in order to save space for keeping larger…

Q: How much solute do you need to make 250 ml of a 3M stock of glucose?

A: The Molarity of a solution is the number of moles of a solute dissolved in a liter of solution. That…

Q: What is the difference of measuring proteins through UV-VIS spectroscopy at 280 nm and at 595 nm…

A: The proteins estimation is a routine process when working with different proteins. The protein…

Q: How will you dilute 100 μl of 25 mg/ml BSA into a final concentration of 2 mg/ml BSA?

A: BSA stands for bovine serum albumin. It has several applications in laboratories. In a microbial…

Q: You have 200 mL of a 1.5 M glucose solution. How many moles of glucose are there?

A: Introduction: Glucose is a monosaccharide and a major source of energy for the living body. The…

Q: The calibration curve shown below was used to analyze an unknown protein solution. What is the…

A: The unknown concentration of a substance in the given sample can be estimated by comparing the…

Q: A patient is suspected of having low stomach acid, a condition known as hypochloridia. To determine…

A: Acids and bases are two chemical substances that react with each other to form a salt and water.…

Q: the following questions about protein concentration determination. a) At what wavelength should an…

A: Spectrophotometry is a process in which the intensity of light is measured, which determines the…

Q: You have a 15% (m/v) solution put any units in your answer, ju

A: Dilution Equation M1 V1 = M2  V2

Q: If equal portions of tretinoin gel, 0.1 % w/w and 0.4 % w/w are combined, what would be the…

A: The objective of the question is to find the resultant percentage strength when equal portions of…

Q: Calculate the unknown concentration of the PROTEIN C with an absorbance value of A412 given the…

A: A linear regression analysis will be required to determine the unknown concentration of Protein C.…

Q: A Biochemist perform different experiments involving ELISA in order to determine the amount of…

A: Introduction Linear equation given as Y=0.9988 x -1.437 where Y=log A (Absorbance) and x=log [C]…

Q: 2. 0.1 mL of a protein solution of concentration of 7 mg/mL was diluted to a total volume of 4.0 mL…

A: The c1v1 formula is a mathematical expression that is used to calculate the relationship between the…

Q: A liter of a TPN solution contains 500 milliliters of 50 percent dextrose solution and 500…

A: To determine the daily energy and protein intakes of a person receiving 2 liters per day of the TPN…

Q: what is the dna concentration suspended in PO4 buffer at ph 7.0 that has an absorbance at 260 nm…

A: DNA concentration of a sample can be determined using spectrophotometry and measuring the absorbance…

Q: if you have Quinoa Serving size -170 grams Carbohydrates – 109 grams calculate the DV % for these…

A: DV% is how much of a nutrient is present in a single serving of a packaged food or a dietary…

Q: Refer to the following table below to answer the succeeding questions: Protein Isoelectric pH…

A: Two dimensional gel electrophoresis is an electrophoretic technique in which in the first dimension…

Q: You are asked to prepare HEPES buffered saline with the following constituents: НЕРES 10mM KCI 5mM…

A: The concentration of a solution is expressed in terms of the ratio of the amount of solute present…

Q: Make 100 ml of a 1% solution of boric acid isotonic with blood by adding NaCl. The freezing point of…

A: A drug, often known as a medicine, is a chemical compound that has a biological effect on the human…

Q: Consider a mixture of two proteins with molecular weights of 20,000 and 200,000. For simplicity of…

A: Sedimentation Coefficient is the rate per unit centrifugal field experienced by the particle…

Q: Figure 3: ● • . ● KDa ● 97.4 66.2 45.0 ● 31.0 21.5 14.4 S-1 p-1 2.8 2-d S.3 p-3 This figure was…

A: The study of protein behavior under different conditions is a fascinating aspect of molecular…

Q: Calculate protein concentration in unknown samples 1, 2, 3: Absorbance of Unknown 1 = 0.541…

A: Given that the standard BSA concentration is 1mg/mL. 10, 20, 30 ,40 and 50 µL of the standard BSA…

Q: is desired to measure the acidity values ​​of two solid fats coming to a food analysis laboratory.…

A: Since 1.2 ml KOH gets used in oil free titration it will be subtracted from volume of KOH used for…

Q: If you had measured the absorbances of your unknown in test tubes that were half the diameter of the…

A: Proteins are composed of twenty standard amino acids that are attached together via peptide bonds.…

Q: Given the following information, calculate the total activity in the undiluted protein sample.…

A: Introduction: A biological sample that contains one or more proteins is referred to as a protein…

Q: 0.0 M sucrose = (4.36 g − 4.6 g)/30 min=0.008 g/min 0.4 M sucrose = (4.59 g − 4.2 g)/30…

A: Percentage weight change can be calculated by the finding out the difference in the initial weight…

Q: You have a stock solution of protein that you would like to dilute. How much stock protein do you…

A: Dilution is a process to lower the concentration the concentration of a solute by the addition of…

Q: If a protein concentration is 1 mg/ml and diluted it by 1/10;what is the final concentration

A: , a 1:5 dilution (or "1 to 10" dilution) entails combining 1 unit volume of solute (the material to…

Q: A spheroidal bacterium with a diameter of 1.0 mm (micrometer, 1 mm = 10-6) contains 25,000 molecules…

A: A spheroidal bacterium are circular or oval in shape which are generally known as coccus (plural…

Q: You have been given a stock solution of dye that has a 10X concentration. You wish to make 750ul of…

A: Dilution is the process by which we can reduce the given concentration of the solution by adding…

Q: If a pediatric vitamin contains 1500 units of vitamin A per milliliter of solution, how many units…

A: A vital nutrient that is fat-soluble and plays a role in many bodily processes is vitamin A.It is…

Q: Consider a crude extract with a mixture of the 5 proteins listed below. Protein A 4.5 pl 160…

A: Ion Exchange ChromatographyIon exchange chromatography can be used to separate proteins that have a…

Q: In an experiment to determine the degree of saturation in 0.43 g of an unknown lipid, the following…

A: The fatty acids in triglycerides can have one or more double bonds in the hydrocarbon chain. Fatty…

Q: Given a stock protein solution with a concentration of 6 mg/ml, determine the protein concentration…

A: Given Values: Concentration of the protein stock solution = 6 mg/ml Volume of the stock protein…

Q: What number of μm are in 1.038 cm?

A: In Biology, the introduction of microscopes formed the foundation for various research. The…

Q: You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule,…

A: Standard calibration curves are plotted by taking concentration of the solution in x axis and…

Q: You have a protein with MW 48kDa. How many grams of protein are in a 200 mL sample at 50 μM?

A: To calculate the amount of protein in grams in a 200 mL sample with a concentration of 50 μM, follow…

Q: Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange…

A: DEAE cellulose resin had some  groups which are positively charged and can bind proteins through…

Q: 1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL…

A: The four types of biological macromolecules are nucleic acids proteins lipids and carbohydrates.…

Q: Laemmli buffer, also referred to as SDS-reducing buffer, is added to each of the samples. It is a…

A: Buffer is defined as any solution that resists small change in its pH. Laemmli buffer is formulated…

Q: Using the data from the standard curve done previously, you record the absorbance of your extracted…

A: A method for calculating an unknown protein sample's concentration is by using a standard curve that…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.