a) Calculate the weight (in gram/ml) of tumour cells in each vial. Show your workings. b) Calculate the glucose concentration in each vial (in mol/ml). Show your workings. c) Based on Table 1, calculate the glucose concentration / 1 gram tumour cells in conditions A, B and C. Show your workings.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
a) Calculate the weight (in gram/ml) of tumour cells in each vial. Show your workings.
b) Calculate the glucose concentration in each vial (in mol/ml). Show your workings.
c) Based on Table 1, calculate the glucose concentration / 1 gram tumour cells in conditions A, B and C. Show your workings.
![For an enzymatic assay, tumour cells are incubated at different concentrations in individual
vials (Table 1). Please note that the final reaction volume differs between the three setups.
Vial No.
A (ml)
В (ml)
C (ml)
H20
1
5
Tris/HCl buffer pH7
1
1
1
Tumour cells (5%
(w/v)) in buffer
1
3
4
Glucose [25mM]
0.5
0.75
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