a) Name one step in the immunohistochemistry protocol described above which you could omit in order to generate the negative control image B?: b) In order to generate a more robust negative control image, what might you use to replace the step identified in your answer to (a) above. (10 words max) [

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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a) Name one step in the immunohistochemistry protocol described above which you could omit in order to
generate the negative control image B?
b) In order to generate a more robust negative control image, what might you use to replace the step
identified in your answer to (a) above. (10 words max) [
Transcribed Image Text:a) Name one step in the immunohistochemistry protocol described above which you could omit in order to generate the negative control image B? b) In order to generate a more robust negative control image, what might you use to replace the step identified in your answer to (a) above. (10 words max) [
Immunohistochemical staining of an ApoE -/- mouse brachiocephalic artery after blocking and subsequent incubation
with a primary and secondary antibody. Image A shows positive and specific staining for an extracellular matrix
molecule. Image B is the negative control image.
A
B
Transcribed Image Text:Immunohistochemical staining of an ApoE -/- mouse brachiocephalic artery after blocking and subsequent incubation with a primary and secondary antibody. Image A shows positive and specific staining for an extracellular matrix molecule. Image B is the negative control image. A B
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