Please asap 4. A biotechnologist carried out an enzymatic assay of the alcoholoxidase enzyme, for this, he coupled the reaction to give rise to acolored compound and be able to measure it in thespectrophotometer. The colored compound was measured at 405nm and the calibration data is shown below.a) Calculate the concentration of the compound at an absorbance of0.20, 0.41, 0.50, and 0.60.b) What type of assay was used to determine alcohol oxidaseactivity? (continuous and discontinuous enzyme assay)(Left is concentration, right absorbance)Concentración (M) Absorbancia0.250.360.440.570.667.19.612.315.118

An enzymatic assay is a laboratory test used to measure the activity of an enzyme or group of…

Answered: 4. A biotechnologist carried out an…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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11) Using the data above, construct Lineweaver-Burk plots. First, graph the inverse of the reaction velocity (rate of reaction) data in column II versus the inverse of the methanol concentration in column I. Then, on the same graph, plot the inverse of the reaction velocity (rate of reaction) data in column III versus the inverse of the methanol concentration in column I
A purified protein sample was used in a reaction, resulting in an activity of 696.7 nmol min-1. The reaction volume was 145.0 µL and the final volume before loading the plate was 1,050 µL. The total reaction time was 4.25 min. The amount of protein used in the reaction was 4.270 µg. Calculate the specific activity of the sample (in nmol min-1 µg-1).
Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.
The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
Concentrations in biochemical systems are often very dilute. Consequently, scientific notation and logarithms are often used to express concentrations. In scientific notation, numbers are expressed as coefficient x 10" To convert a number to scientific notation, proceed as follows: 1. Move the decimal place so that there is one digit in front of the decimal. 2. Account for the moved decimal in the value of x. If the decimal moved to the right, x is negative; if it moved to the left, x is positive. A logarithm is basically an exponent. Unless otherwise indicated, a logarithm is the a of 10". The numbers after the decimal point are significant; the number before the decimal just identify the location of the decimal point for the number. Notice that it is easy to estimate a logarithm from scientific notation; it's the exponent! Logarithms are commonly used to express the concentration of H. The pH is defined as pH= log (In), where the base number is 2.303. The same general rules as logs…
1) You have been asked to make up four 1.5% agarose gels at 30 ml each. Ethidium Bromide is to be added at 0.5 ug/ml and you have a bottle at 10ug/ml. Write down step-by-step protocol of how to proceed. 2. How would you make up one liter of 1X TAE buffer using a 25X stock?
in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45
Answer the following questions: 3) Provide some possible errors% in the experiment of synthesis of cyclohexene 4) Explain unsaturation test with chemical reactions in the experiment of synthesis of cyclohexene |(Ctrl) -
2. Isolation of Eugenol a) Devise a flow chart that summarizes the distillation, extraction, and isolation of eugenol performed in this experiment. b) Would you be able to isolate either ethanol from fermented fruit or R-limonene from the peels of oranges by steam distillation? Briefly explain your answer for each case. H. R-limonene
Pretend the results below were obtained for water from your well. The 10 mL tubes are Full Strength, or 9.9 mL of the sample plus 0.1 mL of 10x broth (here a fluorescent Brilliant Green Broth). The 1 mL tubes are 1 mL sample plus 9 mL of normal broth (so a ten-fold dilution). The 0.1 mL are that much sample with 9.9 mL of the broth (so a hundred-fold dilution). Dark green indicates coliform presence. Use the table and the figure and give me the MPN. To do so, count the number of positive tubes at each dilution. Then match the counts to the set of values that most closely fits. Yes, you need to consider the set of values for None, 1 and 0.1 taken together. For example, 5,2,1. What is the MPN estimate for this well water sample? If safe drinking water is <5 cells/100 mL would you drink this well water?
Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.192 and A650=0.090, taking into account the dilution factor (100), and the total volume of extract (45). Note your answer to 2 decimal places.
What would be the best buffering agent to choose if you wanted to buffer an enzyme reaction or tissue culture medium at pH = 7.95? Group of answer choices a. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid); pK = 7.5 b. Histidine; pK1= 1.78; pK2= 5.97; pK3= 8.97 c. Bicarbonate; pK = 6.1 d. Phosphate; pK1 =2.0, pK2 = 6.8; pK3 = 12.5 e. Tris or Tris(hydroxymethyl)aminomethane; pK = 8.07

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