In DNA replication, the nucleotides used during PCR __________ compared to the nucleotides used during the replication of DNA within a cell.   Question 6 options:   contain the base U instead of T   are somewhat different   are completely different   are exactly the same   contain the base U instead of A

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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In DNA replication, the nucleotides used during PCR __________ compared to the nucleotides used during the replication of DNA within a cell.

 

Question 6 options:

 

contain the base U instead of T

 

are somewhat different

 

are completely different

 

are exactly the same

 

contain the base U instead of A

Expert Solution
Step 1 Introduction

Polymerase chain reaction

PCR, which is a laboratory technique used to amplify a specific region of DNA in vitro. The technique was first developed in the 1980s by Kary Mullis, and it has revolutionized many areas of biological research and medical diagnosis.

The PCR process involves several cycles of heating and cooling a reaction mixture containing the DNA template, primers, nucleotides, and a DNA polymerase enzyme.

During each cycle, the reaction mixture is heated to a high temperature to denature the DNA strands, then cooled to allow the primers to anneal (bind) to the complementary sequences on the template strands, and finally heated again to a moderate temperature to activate the DNA polymerase enzyme, which extends the primers and synthesizes new DNA strands.

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