You have been asked to make up four 1.5% agarose gels at 30 ml each. Ethidium Bromide is to be added at 0.5 ug/ml and you have a bottle at 10ug/ml. Write down step-by-step protocol of how to proceed.

Q: Please provide in detail.

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Q: What methods did the authors use to prove they have the right material?   4,4'-DIBROMOBIPHENYL…

A: The objective of the question is to understand the methods used by the authors to confirm that they…

Q: How do you prepare 50 ml of 20 mg/ml proteinase K from 50 mg/ml stock solution

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Q: Lucas's unwashed, dirty hand produces 947 CFU when swabbed onto a nutrient agar plate, while his…

A: CFU stands for colony forming unit.

Q: Gel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS…

A: Electrophoresis is a method used to separate mixtures of RNA , DNA or proteins according to their…

Q: (a) You decide to purify the 5-HT 2A receptor. The protein has a predicted molecular Mass of 46.54…

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Q: Write the protocol for the periodic acid schiff technique

A:

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Q: What are the mechanisms of samples separation work in Thin layer Chromatography?

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Q: Discuss about the reagents used for agarose gel electrophoresis

A: Gel electrophoresis is used to separate nucleic acid.

Q: Dilution: 10-

A: intoduction   A Serial weakening is a progression of weakenings, with the weakening variable…

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Q: How many microliters of the 500mM colchicine is required for making 1.5mL cell culture with 2mM…

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Q: uring incubation, prepare dilutions of the standard antiserum which constitutes the standard curve…

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Q: You are performing an enzyme reaction that works the same way as your wheat germ acid phosphatase…

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Q: Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. Why do you destain the gel?

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Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

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Q: n SDS PAGE the resolving gel :   is 4%(w/v) acrylamide,PH )6.8   is 6-20% (w/v) acrylamide…

A: SDS PAGE stands for Sodium Dodecyl Sulphate Poly-Acrylamide Gel Electrophoresis. It is a technique…

Q: Sample A: Sample B: Sample C: Sample D: Sample E: Sample F: 22mm 18mm 35mm 30mm 28mm 28mm 21mm 17mm…

A: Introduction The zone of inhibition is the area around an antibiotic pot where bacteria cannot grow.…

Q: 3) Define gel electrophoresis, including its theory and application. Describe the steps of running…

A: Introduction:- Electrophoresis is a biophysical technique mainly used to separate proteins and…

Q: Briefly explain how you could use the color of the Coomassie assay sample solution to determine if…

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Q: You need to prepare medium for your culture cells. Your salt solution is supplied at a 10X…

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Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

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Q: the optical density of your cell culture is 0.92. How much of this  culture should you add to 500 mL…

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Q: Order: Zosyn 0.02 gm/kg Supply: 5 gm powder vial with directions to add 10 mL of sterile water as…

A: Here we have to calculate how much diluent we need to use to reconstitute Zosyn.

Q: Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two…

A: In labs, a centrifuge is a vital instrument that isolates diverse parts of a mixture, like fluids…

Q: How should you prepare your running buffer?

A: SDS-PAGE is method of separation of protein based on its size. Running buffer of the SDS-PAGE…

Q: gel electrophoresis

A: Gel Electrophoresis is a simple , rapid and analytical technique which is used for separating the…

Q: If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not,…

A: CFU  Colony forming unit it a method to calculate the number of microorganisms in a culture media.