You have been asked to make up four 1.5% agarose gels at 30 ml each. Ethidium Bromide is to be added at 0.5 ug/ml and you have a bottle at 10ug/ml. Write down step-by-step protocol of how to proceed.

The Bradford reagent gives a linear response only from 0.1 mg/mL to 1.4 mg/mL of protein concentration. Would we be able to use an "absorbance vs. concentration" line graph for samples outside of the range indicated? Justify your response.

one lm of milk was added to 9 ml of sterile saline. 0.1ml of the diluted sample was put into a petri dish with melted agar. How many cfu/ml are in the milk if 200 colonies were counted?

A urine sample is diluted 1:1,000. If 0.1 ml were put into a Petri dish with melted agar, how many CFU/ml are in the urine if 158 colonies grow?

A culture of S. cerevisea has an overnight OD of 2.3 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies.  How much of the 2.3 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ul

o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the stock solution?

When running a gel, the lane loaded with a negative control sample should will always show a band that lines up with the experimental band, regardless if the sample is contaminated. not show a band, which indicates the sample is contaminated. show a band, which indicates that the sample is not contaminated. not show a band, which indicates that the sample is not contaminated.

This is my data collected from a Succinate Dehyrogenase Enzyme Assay - I am asked to plot a Concentration vs Time while plotting the Resuspended and Supernatant Reaction; how can this be completed? Test Tube Concentration (uM) Absorbance @ 600nm #1 0 0.00 #2 5 0.090 #3 10 0.187 #4 20 0.368 #5 40 0.715 #6 60 1.075     Table 3. Succinate Dehydrogenase Assay @ 600 nm Time point (min): Resuspended Pellet Reaction Absorbance Supernatant Reaction Absorbance Enzyme Reaction Control Absorbance 0 43.2 44.2 46.1 3 43.3 44.8   6 43.0 44.9   9 42.6 45.2   12 43.5 45.6   15 44.0 45.7   18 43.8 45.9   21 44.0 46.4   24 44.1 46.5   27 43.9 46.7   30 43.7 46.9 43.6

Why is necessary to remove fat and tendons from the heart sample in a LDH purification experiment?

Briefly describe how you would make 500 mls of a 1X electrophoresis running buffer from a 15X stock solution.