1. Why is necessary to remove fat and tendons from the heart sample? 2. Why is necessary to completely grind the beef? 3. Why is necessary to balance the homogenate tubes for centrifugation? 4. How do you prepare a 100mL of 0.1 M phosphate buffer? 5. From the anterior buffer, how do you make 100mL of 0.05 M? 6. Calculate the amount of ammonium sulfate necessary to get a 20% solution? 7. Which is the importance of dialysis?
Q: Explain the purpose and phase where cells lose flagella and motility in biofilm formation, and why t...
A: There are several steps associated with the biofilm formation. The first step includes adherence of ...
Q: When did photosynthetic organisms first appeared? O From the very beginning of life, they are some o...
A: Photosynthetic creatures, also called photoautotrophs, are photosynthesis-capable organisms. Higher ...
Q: What is the relationship between plant anatomy and cytology ?
A: Plant anatomy Plant anatomy is the study of plant organ tissue and cell structure. Anatomy, as appli...
Q: application of this principle to straddling fish stocks and migratory fishes
A: CBD = Convention on Biological Diversity
Q: Identify one component of the cell. Describe the structural appearance using your own words Explain ...
A: 1)Cell is the smallest basic structural, functional and biological unit of life.The main components ...
Q: You set up an experiment where you place a sealed (clamped) dialysis tube cell in a beaker of water....
A: Dialysis: it is a process to remove the waste material of the body in the patients of kidney malfunc...
Q: II. Identify the taxonomic rank of the following: 1.Conostyloideae 2.Cucurbitaceae - 3. Hamamelidae ...
A: All plants, animals, and microbes in the world are included in taxonomy, which is the discipline of ...
Q: What should be the main consideration/s in grouping protozoans?
A: In the classification system, protozoa are considered a subkingdom of the kingdom of Protista, despi...
Q: Reproductive modes can be defined based on energetic provisioning (lechithotrophy vs. matritrophy) o...
A: Based on the mode of reproduction , animals can be classified. Based on the type of partition (parit...
Q: Assessment about - citing the Evidences of Evolution
A: The term evolution is associated with variations that can be found among the groups of living beings...
Q: What is apoptosis? How is it beneficial to the body?
A: APOPTOSIS: * Apoptosis is a type of programmed cell death in which some steps in cell will leads to...
Q: why is it important for all somatic cells to be genetically identical?
A: Except for sperm and egg cells, a somatic cell is any cell in the body. Somatic cells are diploid, w...
Q: QUESTION 4 Shown here is a graph illustrating data from the original Pulse-Chase experiment analyzin...
A: The correct answer is- rough ER
Q: How long does it take to transcribe gene
A: Transcription is the process by which RNA Polymerase read out the DNA and synthesized RNA. RNA polym...
Q: an help me to identify this image by labelled them and describes also
A: This histopathological image is of Nodular Lymphocyte predominant hodgkin lymphoma.
Q: Mitosis: 2n = 70 21. Number of chromosomes after Prophase? 22. Number of chromosomes after Anaphase?...
A: Mitosis 2n=70 Number of chromosomes after prophase: 70 Number of chromosomes after anaphase: 70 Num...
Q: What is the specific epithet of sequoia sempervirens (d.don) endl 2. Xylem is mainly responsible i...
A: Sequoia sempervirens: Sequoia sempervirens is the only extant species of the genus Sequoia, which b...
Q: What is the virulence factor of Mycobacterium tuberculosis
A: Virulence factors are specific molecules of pathogenic organisms that cause the invasion against the...
Q: As a rule of thumb, each 1% of soil organic matter by weight contains about 1,000 pounds of nitrogen...
A: Any material created by living organisms (plants or animals) that is returned to the soil and decomp...
Q: Identify the type of fluid or the fluid compartment in which the given item would be found. Gamma Pl...
A: Intracellular fluid (ICF) is the cytoplasm of the cell. Extracellular fluid (ECF) is the medium that...
Q: Can u give 5 Statement of the problem about this topic thank you Topic: Implication study of vermic...
A: When organic manure is produced by the use of earthworms, which generally leaves in soil, eats bioma...
Q: Give a biologic example of the negative feedback loop?
A: Introduction :- A self-regulating system is a negative feedback loop, also known as an inhibitory lo...
Q: To map the location of a tumor in the brain, a neuropsychologist uses a/an ______, which creates a t...
A: Introduction: When cells grow and divide more than they should or do not die when they should, an ab...
Q: 15. On the tree below, mark the place or places that three tissue layers have evolved in animals. Ti...
A: ANSWER;- Homology is sharing of common ancestry and exhibiting similarity of structure and physiolog...
Q: Please answer fast 1. Fertility can be compromised in women when their body mass index (BMI) falls ...
A: The prenatal development process is divided into three stages. The germinal stage lasts for the firs...
Q: Comprehensive explanation about how the species of finches , that were found by darwin at galapagos ...
A: Natural selection, according to Charles Darwin's theory of evolution, is how evolution occurs. Physi...
Q: aerobic and anaerobic respiration, NADH is oxidized to return to NAD+ which is needed for Glycolysis...
A: Aerobic respiration can be described as the process involving the breakdown of carbohydrates such as...
Q: Match the numbers with the correct structure names 1 aorta tricuspid valve left ventricle
A: Heart: human heart has four chambers as follows- right atrium, right ventricle, left atrium, and lef...
Q: Perichondrium is
A: Perichondrium is layer of connective tissue that surrounds the cartilage of developing bone. It con...
Q: What is the significance of mapping the human genome?
A: Human genome : It is a complete set of nucleic acid sequences for humans , encoded as DNA within the...
Q: Imagine you are working in a genetics lab with the fruit fly Drosophila melanogaster, a model organi...
A: Fruit flies are tiny insects that eat fruits and vegetables. Its scientific name is Drosophila. The ...
Q: АCTMTY 10.6.1 Label the diagram and explain the salient feature that is happening in each item. For ...
A: Introduction: Electron Transport Chain is a series of compounds where it makes use of electrons from...
Q: 1. A short-day plant flowers only when exposed to light for less than 12 hours long. Which of the fo...
A: Short day plants When the days are shorter than their critical day length, these plants begin to bl...
Q: 4. If several pea plants with the genotype TTYY are crossed with pea plants with the genotype Ttyy, ...
A: There will be two heterozygous plant crosses as a result: T T T TT TT t Tt Tt Y y...
Q: What is the name of the LT receptor which recognize the hla molecules of the graft
A: Introduction:- The human leukocyte antigen (HLA) system (also known as the major histocompatibility ...
Q: Gene expression is... How genes are replicated into two new strands identical to each other How gene...
A: Gene expression is the process in which DNA is transformed into a functional product such as Protein...
Q: 1. Consider a locus having two alleles Aj and A2, If the homozygote A1A1 and the heterozygote A1A2 h...
A: Introduction :- The relative frequency of an allele at a specific locus in a population, expressed a...
Q: n what ways can cellular growth and division be disturbed?
A: Cell division or cellular regulations is a normal cellular process that takes place in all living th...
Q: Explain why in human families, many traits (albinism, phenylketonuria, blue eyes ) skip a generation...
A: Introduction :- Phenylketonuria, or PKU, is a rare hereditary condition in which the amino acid phen...
Q: Asap
A: The analytical method is a technique for analysing a fact, a sample, or something similar. These are...
Q: How does a rib articulate with a thoracic vertebra?
A: The skeletal system is usually defined to stated that they are been made up of all the bones and joi...
Q: Which one of the following would upregulate a gene? Activator transcription factors O DNA Polymerase...
A: Gene regulation is crucial for viruses, prokaryotes, and eukaryotes because it enhances an organism'...
Q: Use the dichotomous key in Table 2 to identify a gram-negative rod that ferments lactose and uses ci...
A: Bacteria are ubiquitous, mainly free-living organisms with only one biological cell. They are indeed...
Q: PHow does the oxygen binding curve of fetal hemoglobin differ from that of adult hemoglobin
A: Introduction: Haemoglobin is an iron-containing oxygen transport metalloprotein found in all vertebr...
Q: Give short description to explain how motor coordination and balance are regulated.
A: A nervous system is defined as an organized group of cells called neurons that are specialized for t...
Q: glutamate when it binds to postsynaptic AMPA receptors
A: glutamate is the main excitatory neurotransmitter in the central nervous system, it primarily intera...
Q: occurring
A: The Department of agriculture regulates field testing of GM crops for research. The environmental pr...
Q: Which of the following exhibits Invoked Organization? * i. Regal Angelfish ii. Orb web spiders iii. ...
A: Although each living and non-living entity in the world appears to follow its unique pattern, a clos...
Q: Unlike a rabbit, running all-out for a few moments to escape a predator, migratory birds require ene...
A: Acetyl coenzyme A is a key metabolic intermediate that is produced in a variety of methods, includin...
Q: What is the morphology of the following cells: sarcina tetrad diplococci ...
A: Bacteria are ubiquitous microscopic prokaryotic organisms which neither possess true nucleus nor mem...
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- 1. (2.5 pts) A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 1 2 3 5 423 80 13 Number of colonies. A pharmacist had 6 grams of codeine sulfate. He used it in preparing the following: 12 capsuleseach containing 0.0435 gram, 14 capsules each containing 0.145 gram and 23 capsules eachcontaining 0.011 gram. How many milligrams of codeine sulfate were left after he had preparedthe capsules?A pharmacist had 6 grams of codeine sulfate. He used it in preparing the following: 12 capsules each containing 0.0435 gram, 14 capsules each containing 0.145 gram and 23 capsules each containing 0.011 gram. How many milligrams of codeine sulfate were left after he had prepared the capsules?To prepare a vaginal irrigation, a nurse uses 3 tsp of vinegar and 1.5 L of water. a. How many ml of vinegar should she use? b. If she prepares a solution using only 500 ml of water, how many ml of vinegar should she use?
- Now prepare a 500 ml media having ¼ strength MS with 5mM BAP, 5.5 mg/l Kn and coconut water 10%. Stocks are MS Macro 10X, micro 20X, FeEDTA 10X, Organic 100X, BAP and Kn 5gm/100ml. If you need anything else then you add accordingly. BAP has a MW of 225.3. Sucrose and Myo-inositol is not mentioned in the question. These you need to add. Consistency of the medium is also mentioned.Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:1. give other urinary constituents which may reduce benedict’s qualitative solution other than glucose. 2. What Substances Give The Same Reaction As Sugar With Nylander’s Test And How Do You Remove It? 3. by what other method can reducing sugars be differentiated from each other and from glucose? 4. what substances interfere with the tests for fructose? for lactose? 5. what sugar causes the same color reactions with the test for pentoses? why? 6. what other substances interfere with the indican reactions? 7. Why should the specimen be albumin free when performing the chloride test for urine?
- Where do you expect to see the highest number of CFU on a plate: Select one: a. When pasteurized milk is inoculated at dilution of 10-8 b. When pasteurized milk is inoculated at a dilution of 10-2 c. When raw milk is inoculated at a dilution of 10- d. When raw milk is inoculated at a dilution of 10-8 e. When raw milk is inoculated directly at a dilution of 10-250 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then a 1:90 dilution is made from this, and then 1 mL from this is added to 99 mL of water, and then 100 uL of this last dilution is plated and after incubation 164 colonies are counted.a) What was the overall dilution factor?b) What was the total CFU/g of the original sample?1) If you mix a starch solution with freshly collected saliva and incubate it for 30 minutes at 37°C, will this mixture test positive for the IKI test? Why or why not? 2) If you mix a starch solution with saliva and incubate it for 30 minutes at 37°C, will this mixture test positive for the Benedict test? Why or why not? 3) Bile works as a biological emulsifier that emulsifies lipids in the small intestine. Emulsifiers are often used in the food industry. How can one tell if an oil and vinegar salad dressing has an emulsifier in it? 4) What are the products of the chemical digestion of triglycerides by pancreatic lipase? 5) What type of pH environment is most optimal for pepsin to digest proteins? 6) What is the difference between Pepsin and Pepsinogen? 7) Which of the following digestive enzymes is not present in the oral cavity? A) lipase B) amylase C) trypsin D) all of the above 8) Where is the enzyme lactase normally produced? A) intestine B) pancreas C) saliva D) stomach 9) Which…
- What will be the color of saliva extraction with iodine in 3, 6 9, 12, 15, and 18 mins if: you put 5 ml of starch soln. and you heat it to boiling after that you will add 1 ml of saliva. Cool and then continue to heating after that you will place it in a water bath at 60 degree Celsius. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results.After performing a plate and liquid lysate, it was found that the plate lysate achieved the common titre range of 1010-1011 pfu/ml whereas the liquid lysate achieved a range of 108 PFU/mL; what is the reason for the lower titre in different methodologies? How can we troubleshoot this issue in the future?From a 5% normal saline (NaCl) solution, take 2 ml and dilute it 1:5. Take 5 ml of this solution and dilute it 1:10. A 2:100 dilution is made from this last solution. Determine: a) How many times less concentrated is the 2nd dilution than the original? b) What is the dilution factor in each step of the series and what is the final volume of each dilution? c) How many grams of NaCl will be in 1 ml of the last solution?