1- Are the following changes in conditions likely to increase or decrease retention time?a. faster flow rateb. longer columnc. longer connector between column and detectord. higher analyte concentratione. higher temperaturef. larger sample sizeg. using a less polar column while doing a reverse-phase separationh. using a less polar mobile phase while doing a reverse-phase separation2- In what order will the following substances elute?• In reverse-phase liquid chromatography:a. butylamine, 2-butylene, ethylamine, ethyleneb. benzene, chlorophenol, phenol, trichlorophenol• In gas chromatography with a polar column:c. butanol, methanol, water• In gas chromatography with a nonpolar column:d. butanol, methanol, water• In ion chromatography:e. bromide, fluoride, sulfide

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1- Are the following changes in conditions likely to increase or decrease retention time? a. faster flow rate b. longer column c. longer connector between column and detector d. higher analyte concentration e. higher temperature f. larger sample size g. using a less polar column while doing a reverse-phase separation h. using a less polar mobile phase while doing a reverse-phase separation

1- Are the following changes in conditions likely to increase or decrease retention time? a. faster flow rate b. longer column c. longer connector between column and detector d. higher analyte concentration e. higher temperature f. larger sample size g. using a less polar column while doing a reverse-phase separation h. using a less polar mobile phase while doing a reverse-phase separation

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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