6)1 mL of supernatant is required for a procedure. The final colored solution proves to be too highto read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilledwater are substituted for the original supernatant and the procedure, run as before. The readingfrom the standard curve is 46 mg/dL. What is the actual amount of substance in the patientserum?

Introduction: The concentration of a solution is an estimation of the amount of solute that is…

Answered: 1 mL of supernatant is required for a…

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter15: Instrumentation In Medical Imaging

Section: Chapter Questions

Problem 7P

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Similar questions

In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?
A test procedure calls for 5 mL of undiluted specimen. 2 mL of a 1:50 dilution is used. The answer obtained is 30 mg/dL. What answer should be reported?
An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?
The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?
What is the estimated size of the specimen under a 10x10 ocular micrometer under HPO? (Take note: specimen Occupies 10 OU) Where: 1 OU = 25µ
To determine the cfu/ml of a pondwater sample, you perform 1/10, 1/100, 1/1000 and 1/10,000 serial dilutions and plate 0.1 mls of each dilution. You count 40 colonies on the plate derived from the 1/10,000 dilution. What is the cfu/ml of the pondwater? Show your work and use scientific notation.
After placing sample in this instrument, what are the names of two fractions in the tube (choose all that are correct)? ruer O Pellet OBase O Supernatant OFluid O Precipitation
The BSA stock solution from the previous problem was then diluted to generate a set of standard solutions of known concentrations. After performing biuret assay on these solutions, their absorbance at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained. Concetration of BSA Absorbance (mg/mL) 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8 0.380 1.0 0.485 Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.
Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:
How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of sample and volume of dilute would be used?
If you plate 200 ul of a 1:100,000 dilution and get 123 colonies, what is the number of CFU/mL in the original sample?
Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the distance of one ocular division? Show your solution.

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