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GQ1
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- 1. Identify the name of the correct amino acid that corresponds to the correct tRNA codon and mRNA codon. State the process used to convert to mRNA and tRNA.Q1: In your own words, define RNA splicing. When during gene expression does it occur? Q2: What do you predict would happen if the introns were not removed from RNA before translation? Why would it be a problem if the introns were not removed? Q3: Where is the mRNA destined to go once it has been transported out of the nucleus?Describe the roles of RNA molecules in gene expression.
- 1. DNA is a linear code of biological information in the form of genes. DNA is transcribed to another linear molecule RNA, which is translated into a linear polypeptide. For each gene, there sites which control the transcription and translation and sites which defines the beginning and end of the gene, the mRNA and the polypeptide. For a single gene, its mRNA and the resulting polypeptide, draw all the three molecules in parallel and indicate the control sites and those which mark the beginning and end of each.1.Describe the journey of a protein, from its synthesis to its final destination 2. Explain the different stages of transcription and the role of the elements involved with the right terminology (template vs. non-transcribed strand, promoter, stop sequence, TATA box, general and specific transcription factors, RNA polymerase II, etc.); 3. Explain what the maturation (modifications) of pre-messenger RNA consists of and its role6. How many amino acids will the mRNA sequence "AUG GÁC CUG UCG UGA" produce? (LS1-1) * Second mRNA base G UUU Phe UUC UCU UAU UGU Tyr UAC Cys UCC Ser UCA UGC C Phe Gly (G) Leu UAA Stop UGA Stop A Glu (F) (L) UUA Leu UUG Ser Asp (E) (D) (S) Tyr (Y) UCG UAG Stop UGG Trp GUC Ala CUU (A) A CCU CAU CGU His CUC Leu CUA CC Pro ССА CAC CGC Arg CGA Cys (C) Val CAA Gln CAG G U (V) G Trp (W) CUG CCG CGG Arg (R) G U A C A C Leu AUU ACU AAU Asn AGU Ser AGC (L) Ser (S) C AUC Ile ACC AAC Thr Lys (K) UG A AUA Pro AGA Arg AGG ACA AAA Lys AAG Asn (N) (P) AUG Met or start ACG His Thr (H) (T) Gln GUU GCU GAU Asp GAÇ GGU (Q) lle Arg (R) (1) GUC Val GUA GCC Ala GCA GGC Gly GGA GAA Glu OGAG GUG GCG GGG O 1 3 4 First mRNA base (5' end of codon) - U A GUca Gbc AG Third mRNA base (3' end of codon)
- 1. What mRNA sequence is synthesized from a section of DNA that is 3’-TTGACCT-5’? 2. In what direction does a polymerase move when synthesizing a strand of mRNA? 3. Define transcription and translation. Which process occurs first to make protein from DNA?29. pre-MRNA transcript 5' 3' Open boxes represent introns Filled boxes represent exons A) Draw the MRNA to be translated if the pre-mRNA is constitutively spliced. S B) Draw an mRNA to be translated if the pre-MRNA is alternatively spliced due to exon skipping.Which of the following statament is NOT TRUE about gene expression? Lüffen birini seçin: O a. Gene expression is the process by which DNA directs the synthesis of proteins O b. During gene expression, the information encoded in genes is used to make specific polypeptide chains or RNA molecules. O c. The expression of genes that code for proteins includes two stages: replication and translation O d. Translation is the synthesis of a polypeptide using the information in the MRNA.
- 1. Identify the major types of RNA in a cell and discuss their individual functions in relation to protein synthesis. 2. Compare the structural differences of sugars and bases in DNA and RNA molecules 3. What processing events differentiate eukaryotic mRNA from prokaryotic mRNA?8. Label each figure with what TYPE OF CELL is undergoing gene expression: Nuclear membrane DNA Cytoplasm Plasma membrane Nucleus Cytoplasm Mature mRNA Pre-MRNA MRNA DNA Protein Ribosomes Protein Ribosomes4e. You also study the expression of 3 different mutants for this gene. For each mutant answer the following: Does this mutation change the sequence of the protein produced? Why or why not? If it does change the sequence of protein be sure to write out the new sequence. If it does not change the protein sequence, what effect (if any) would you expect it to have on expression of the gene? 1 20 ORI 40 60 5'..TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTATC...3' 3'..AAGCTCGAGAGCAGCAGCTCTATGCGCTACTATAATGACCATTATACCCCTACGTGATAG...5' promoter