1. how to isolate 26s rRNA from water, surface of water and kelps surface 2. what is the PCR tool to use for 26s rRNA. 3. what is the background of the title: metabarcoding of yeast communities associated with kelp beds in marine ecosystem
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1. how to isolate 26s rRNA from water, surface of water and kelps surface
2. what is the PCR tool to use for 26s rRNA.
3. what is the background of the title: metabarcoding of yeast communities associated with kelp beds in marine ecosystem
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- 1. What is the difference between an iterated blast (psi-blast) search and a simple blastsearch?2. Arslan sequenced a gene in lab how he will know about the gene either it is noveldiscovery or gene is already present in the database?3. Ruwaifa want to compares a nucleotide query sequence what option he will opt inBLAST and why?4. Uzair has two protein sequences, he want to check their similarities what he will do? What results are expected1. Why do you think yeast is the one used for RNA extraction?2. Cite qualitative tests to characterized isolated RNA.OF intorcat A Intorest gone in a gene "gun," a bacterial material into the genetic material inserts itself into the chromosomes of the host plant. Engineers must also insert a "promoter" gene from a virus as part of the package to make the inserted gene express itself. . This process alone, involving a gene gun or a similar technique, and a promoter, is profoundly different from conventional breeding, even if the primary goal is only to insert genetic material from the same species (Hansen, 2000). host plant cells. Activity 4: Desirable Traits Directions: Study the plants and animals below that have desirable or enhanced traits. Explain how each of the characteristics was introduced or developed (i.e., classical breeding or recombinant DNA technology). MODIFYING TECHNIQUE (Classical breeding/ Recombinant DNA technology) REASON ENHANCED TRAIT 1. Kobe / Wagyu Beef (Beef with good fat distribution)
- 6. Explain why a positive test for COVID19 would appear sooner than a negative result when using real- time PCR to test if someone is infected. 7. Explain why the same primers (GMM) could detect several types of genetic modifications to common crop plants, rather than one specific gene or genetic modification. 8. Explain the general procedure for using the NanoDrop machine in analyzing DNA samples. Be sure to explain the purposes of the blanks (pure water), and of the importance of the 260/280 ratio. Identify an ideal 260/280 ratio of purified DNA.18. You want to express hemoglobin beta (HBB) in a bacteria model using a vector. You would like to amplify the following gene by PCR. GTAGAATATG ATAAGCGAAA CTGCAAATCG CGTTTGGGGC GATACAAGTA GTGTACGCGG ACCGCGCCGA GCGGGCTATG GTTTCTCCAC TATCAGTTCT TCTCCTGTCC CAGCGAAGTC GAGGTCCAGC CCTACGGTAC ATAACTAACA CGGTTTGAAG AAGATACGAT CTTACGAAGT AAAGAAAATT TGTAGTCAGC CCGGTTCGCT TGTGTCCAGC TAATCGATTG ATTGGCCCCA GCAGGCGAGA TGAACATAGT CATGCGCTGT CTAATAGCCC ATTTGACGTG TAGGTGGCGC TTTTATTTCT GAGGTGGAAA T a) If the primers were both 20 nucleotides long, what are the sequences for both PCR primers that will amplify only the highlighted region? Be sure to label the 5' and 3' ends. (4 points) b) What is the length (in bp) of the entire region amplified by their primers (i.e. the amplicon length)? (Note: the spacing in the above sequence is intentionally uniform) (2 points) c) If you start with 300 copies template DNA how many copies would you expect to produce if you ran the PCR for 22 cycles? Show your work.…Metagenomics has revolutionized our understanding of the microbial world by allowing the study of organisms that had been impossible to culture. Understanding the Lokiarchaeota required metagenomics analysis, which has also led to the identification of the eukaryotic signature genes in other environments. The terms below relate to genes and genetic analysis. Drag each term to the correct description. Drag and drop the terms on the left to match the description on the right. ▸ View Available Hint(s) Submit Homologous Monophyletic Metagenomics Orthologs Paralogs and that has a different function (this occurs through gene duplication) : the study of genetic material from an environmental sample Reset : a group of organisms in a phylogeny that have a common ancestor : a gene that is related in different species by being inherited from a common ancestor Help and that has the same function : a gene that is related in different species by being inherited from a common ancestor : a gene that…
- Why is gene editing becoming a trend in modern genetic engineering? Provide a concrete example on your explanation. 2. Out of 4 presented methodologies in gene modification/editing, which do you think is the most promising in providing efficient result? Why do you say so? 3. What makes the species of Agrobacterium ideal for genetic engineering? Describe its characteristics and its role in producing transgenic plants. 4. In which of the following aspects do you think it is worthwhile to develop genetic engineering? Why or why not? a.) Agriculture and Food Industry b.) Medicine c.)Research d.) Entertainment 5. What are the possible bioethical issues that gene editing tools may encounter? 6. Do you think genetic engineers play God when they modify the genes of various organisms to enhance their existing traits? Why or why not?Part 2. PCR 1) In one color, write out the forward primer (5’ GATAC 3’) in the correct position relative to the given template DNA sequence. In a second color, act as the polymerase and fill in the rest of the new strand of DNA Primer/New Strand Template DNA: 3’ TAGCTATGCGGACCTCATGCATTAGAGTAG 5’ Part 3. Restriction Enzymes 1) Consider the sequence of DNA given below and answer the following questions 5’ ATTGAGGATCCGTAATGTGTCCTGATCACGCTCCACG 3’ 3’ TAACTCCTAGGCATTACACAGGACTAGTGCGAGGTGC 5’ a) You cut the sequence of DNA shown above using BamHI (see table 19.1 from the text). How many fragments of DNA would you expect to result from this restriction digest? b) If you cut the sequence of DNA shown above using BclI (recognition sequence = 5’ TGATCA 3’, enzyme cuts after the first T) instead of BamHI how many fragments do you expect? 2) For each given sequence/restriction enzyme pair, determine how many pieces of DNA would result form the digest and…2. You have been given a DNA fragment obtained from the genome of Bacillus thuringiensis below: 3'CACTAACTGTCGCCAGGTCTGATAGACATATAACTGTTGGCGTACATAAGAAGG АТСАААААА5 Additional tools are provided below. Angela Parry-Hanson Kunadu & Dr. Joycelyn Quansah Page 1 of 1
- 23. Important elements of a directed evolution experiment (“evolution in a test tube”) for an ATP-binding aptamer include: MARK ALL THAT APPLY. Group of answer choices Mutagenic PCR DpnI restriction enzyme Randomized DNA pool ATP affinity column1. Below are the agarose gel electrophoresis and spectrophotometric measurement results (RNA concentration value and A260/A280 to A260/A230 ratios) of total RNA samples isolated from 4 different eukaryotic cells. 1. Explain the agarose gel image and spectrophotometer results of the 1st, 2nd, 3rd and 4th group RNA samples. 2. Are the 1st, 2nd, 3rd and 4th group RNA samples obtained pure? If it is not pure, write what kind of contamination, if any, is present in the RNA samples. 3. If there is contamination in the RNA samples belonging to the 1st, 2nd, 3rd and 4th groups, explain how they are eliminated. 4. Explain the reason for the contamination in the contaminated samples, which may have resulted from the error or deficiency in which step of the RNA isolation1. Explain why the 16S rRNA gene sequencing is suitable for bacterial identification in general and why it is mostly limited to genus level.