1. Below are the agarose gel electrophoresis and spectrophotometric measurement results (RNA concentration value and A260/A280 to A260/A230 ratios) of total RNA samples isolated from 4 different eukaryotic cells. 1. Explain the agarose gel image and spectrophotometer results of the 1st, 2nd, 3rd and 4th group RNA samples. 2. Are the 1st, 2nd, 3rd and 4th group RNA samples obtained pure? If it is not pure, write what kind of contamination, if any, is present in the RNA samples. 3. If there is contamination in the RNA samples belonging to the 1st, 2nd, 3rd and 4th groups, explain how they are eliminated. 4. Explain the reason for the contamination in t

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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1. Below are the agarose gel electrophoresis and spectrophotometric measurement results (RNA concentration value and A260/A280 to A260/A230 ratios) of total RNA samples isolated from 4 different eukaryotic cells.

1. Explain the agarose gel image and spectrophotometer results of the 1st, 2nd, 3rd and 4th group RNA samples.

2. Are the 1st, 2nd, 3rd and 4th group RNA samples obtained pure? If it is not pure, write what kind of contamination, if any, is present in the RNA samples.

3. If there is contamination in the RNA samples belonging to the 1st, 2nd, 3rd and 4th groups, explain how they are eliminated.

4. Explain the reason for the contamination in the contaminated samples, which may have resulted from the error or deficiency in which step of the RNA isolation

1 2 3 4 M
A
Örnek
RNA
A260/A280 A260/A230
1000 bp
900 bp
800 bp
700 bp
600 bp
500 bp
400 bp
300 bp
200 bp
Konsantrasyonu
1
26 ng/µl
1.5
1.4
2
120 ng/µl
1.90
1.7
3
200 ng/µl
1.85
1.8
4
150 ng/µl
1.90
1.8
Transcribed Image Text:1 2 3 4 M A Örnek RNA A260/A280 A260/A230 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp Konsantrasyonu 1 26 ng/µl 1.5 1.4 2 120 ng/µl 1.90 1.7 3 200 ng/µl 1.85 1.8 4 150 ng/µl 1.90 1.8
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