18. You want to express hemoglobin beta (HBB) in a bacteria model using a vector. You would like to amplify the following gene by PCR. GTAGAATATG ATAAGCGAAA CTGCAAATCG CGTTTGGGGC GATACAAGTA GTGTACGCGG ACCGCGCCGA GCGGGCTATG GTTTCTCCAC TATCAGTTCT TCTCCTGTCC CAGCGAAGTC GAGGTCCAGC CCTACGGTAC ATAACTAACA CGGTTTGAAG AAGATACGAT CTTACGAAGT AAAGAAAATT TGTAGTCAGC CCGGTTCGCT TGTGTCCAGC TAATCGATTG ATTGGCCCCA GCAGGCGAGA TGAACATAGT CATGCGCTGT CTAATAGCCC ATTTGACGTG TAGGTGGCGC TTTTATTTCT GAGGTGGAAA T a) If the primers were both 20 nucleotides long, what are the sequences for both PCR primers that will amplify only the highlighted region? Be sure to label the 5' and 3' ends. (4 points) b) What is the length (in bp) of the entire region amplified by their primers (i.e. the amplicon length)? (Note: the spacing in the above sequence is intentionally uniform) (2 points) c) If you start with 300 copies template DNA how many copies would you expect to produce if you ran the PCR for 22 cycles? Show your work. (4 points)

what is the function and origin of this DNA sequence (use BLAST and NEBcutter)? ATGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGAAG ATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAACCCTGGA AAAAGCAGCAGTTGAAGCGTTTAAACTCGGTTACGAAGTGACCGATCCAGAAGAGCTGGAAGTTGAAGAT GGTGATATCGTGATTTGCTGCGACATCCTCAGCGAGTGCGCGTTGAATGCCGATCTGATCGATGCCCAGG TTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGA TCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAA

the topic is Identification of Differentially Expressed Genes in Breast Cancer Using RNA-Seq write a method section of a scientific report for this topic. including the use of excel to get these result( will be included in the images) and also this website https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE216238

The image below shows the general structure of a gene on a chromosome. The arrows above and below the chromosome indicate the binding positions of potential forward (F) and reverse (R) PCR primers. Select two primers from the list below that would exclusively amplify exon 3 in a PCR reaction. ut of Intron 1 Intron 2 +1 Poly-A signal ATG TAG F1 Exon 1 F4 Exon 2 F6 Exon 3 R6 R4 R3 R2 R1 Promoter Select all that apply: cross out Da. F1 cross out Ob. F2 cross out Oc. F3 cross out O d. F4 cross out O e. F5 cross out f. F6 cross out g. R1 cross out Oh. R2 cross out OL R3 cross out O. R4 cross out k. R5 cross out R6 TI

● Gene lacZ fliE Please fill in the table below for the desired mutations from section five. Please reword any pathway information you find and reference the source (not just ecocyc.com) omp A lexA Size of gene (bp) 3075 315 rpoA nagC 1221 990 1041 609 Protein Pathway Expected size of PCR (bp) name

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The following chromatogram is obtained from the sequencing reaction. Using the key below, what message did you integrate into the yeast genome? AATATTAAAGTATAGAACGACTGTGAA A B с D E F G H I AAA AAT AAG AAC ATT ATG ATC AGT AGG J K L M N O P Q R Key GGG GGA GGT GGC GAA GAC GAT GTT GTA S T U V W X Y Z (SPACE) TTT TTC TTA TTG TGT TGA TGG TAA TAG

The sequence of a template DNA is GGGCCATTCGAACGTCCGAAAATGCCCCTGAATGAAAATTTTGGCCC. The sequence of a primer used for PCR amplification of the above DNA is CCCGGTAAGCTT. Where is the 5' end for the template and primer, respectively?   answer options: 1) Left, left 2) Right, left 3) Left, right 4) Right, right 5) Right, left

The sequence below shows 140bp of a double-stranded DNA sequence coding for histone H2A. What two primers could be used to amplify the whole 140bp sequence in a PCR reaction? 1                                                                   70ATGTCTGGACGTGGCAAGCAGGGAGGCAAAGCCCGCGCTAAGGCCAAGACTCGCTCTTCTAGGGCCGGTCTACAGACCTGCACCGTTCGTCCCTCCGTTTCGGGCGCGATTCCGGTTCTGAGCGAGAAGATCCCGGCCAG 71                                                                 140 TCCAGTTCCCCGTGGGCCGAGTGCACCGCCTGCTCCGCAAAGGCAACTATGCCGAGCGGGTCGGGGCCGGAGGTCAAGGGGCACCCGGCTCACGTGGCGGACGAGGCGTTTCCGTTGATACGGCTCGCCCAGCCCCGGCC   a. TACAGACCTGCACCGTTC and GGCCGGGGCTGGGCGAGC   b. ATGTCTGGACGTGGCAAG and CCGGCCCCGACCCGCTCG   c. ATGTCTGGACGTGGCAAG and GCTCGCCCAGCCCCGGCC   d. TACAGACCTGCACCGTTC and CGAGCGGGTCGGGGCCGG

Competent E. coli cells were transformed with the pGLO plasmid. These transformed cells were then allowed to grow on two different plates: 1) a plate containing LB/AMP and 2) another plate containing LB/AMP/ARA. In which plate would you observe both phenotypic and genotypic changes? Briefly justify your answer. Edit View Insert Format Tools Table 12pt v Paragraph v BIUA e T?v 田 D2