18. You want to express hemoglobin beta (HBB) in a bacteria model using a vector. You would liketo amplify the following gene by PCR.GTAGAATATG ATAAGCGAAA CTGCAAATCG CGTTTGGGGC GATACAAGTA GTGTACGCGGACCGCGCCGA GCGGGCTATG GTTTCTCCAC TATCAGTTCT TCTCCTGTCC CAGCGAAGTCGAGGTCCAGC CCTACGGTAC ATAACTAACA CGGTTTGAAG AAGATACGAT CTTACGAAGTAAAGAAAATT TGTAGTCAGC CCGGTTCGCT TGTGTCCAGC TAATCGATTG ATTGGCCCCAGCAGGCGAGA TGAACATAGT CATGCGCTGT CTAATAGCCC ATTTGACGTG TAGGTGGCGCTTTTATTTCT GAGGTGGAAA Ta) If the primers were both 20 nucleotides long, what are the sequences for both PCR primers that willamplify only the highlighted region? Be sure to label the 5' and 3' ends. (4 points)b) What is the length (in bp) of the entire region amplified by their primers (i.e. the amplicon length)? (Note:the spacing in the above sequence is intentionally uniform) (2 points)c) If you start with 300 copies template DNA how many copies would you expect to produce if you ran thePCR for 22 cycles? Show your work. (4 points)

PCR (Polymerase Chain Reaction) is a laboratory technique used to amplify specific regions of DNA.…

Answered: 18. You want to express hemoglobin beta…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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PUC18 (Figure 2) is a plasmid cloning vector commonly used with Escherichia coli (E. coli). Eco01091 2674 Plol 46 BstAPI 179 Aatil 2617 Sapl 2501 Ndel 183 Ehel 235 Pdml 2204 MCS lacz 146 Bogl 2215 Scal 2172 2486 PUC18 2686 bp Bsaxi 659 Sapl 683 AlMI, Pscl 806 Gsul 1784 1626 Cfriol 1779 Eco311 1766 Eamt 1051 1094. 1466 rep (PMB1) \BseYl 1110 \Cail 1217 Hincl Ctr Eci136 Psti Acc65 EcoRI Eco24 Saci Sall EcosBi Xapl 455 M13puC sequencing prier (20). 17mer 399 Hindi SG TAA AAC GAC GGC CAG TGC CAA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CC GGG TAC CGA GCT CGA ATT CGT 3'CATT TIG CTG CCG GTC ACG GTT CGA ACG TAC GGA CGT CCA GCT GAG ATC TCC TAG GGG CCC ATG GCT CGA GCT TAA GCA Lacz -Val val Ala Leu Ala Ser Ala His Arg Cys Thr Ser Glu Leu Pro Asp Gly Pro Val Ser Ser Ser Asn Thr Pael Sdalvel Bam Smal Kont Xmil Xbal AAT CAT GGT CAT AGC TGT TTC CTG 3 TTA GTA CCA GTA TCG ACA AAG GAC S le Met Thr Met MSpic reverse sequencing primer (2). 17 mer Figure 2 X1 gene was inserted at the HindlII and EcoRI…
1a.The complete genotype of the host yeast strain is MATa ade2 his3 leu2 met15 ura3. Name one advantage of using a yeast strain with auxotrophies for several genes (i.e. his3, leu2 and ura3). b.)After successful CRIPSR editing, the yeast can later be “cured” of the recombinant CRISPR plasmid – that is, the plasmid is lost but the CRISPR edit is stably inherited. Write the new genotype of the yeast based on ADE6 gene.
The sequence shown in Figure 2 is the nucleotide sequence of a heat shock protein of Bacillus anthracis obtained from the NCBI database. >NC_005945.1:2107825-2108262 Bacillus anthracis str. Sterne chromosome ATGCGTAATTTATTTCCAGAAATAACAAAACGTCAAAATGGTATTTTTGATTTTGGACCTTCTTTATTAG AAGGGATGACAGATGCTTTCTTTAAACCGATGAACATGGATATTTTTAAAGTAGATGTTCATGAACAATC TGATAAATATACAGTGAAAGCAGATTTACCAGGTTTTCAAAAAGAAAACATTCAAGTTGAATTTGAACAA GATGTATTAACGATTCAAGCAACTAATCATAATGAAGTAGAAGAAAAAAATGAGAATGGCACATATATTC GTAAAGAACGTTCTATAGGTTCTGTAACTCGACGTTTTAGTTTTAAACAAGTTGAGGAAGAAAATGTTAG AGCGAATTACAAAGATGGCGTGTTGACAATTGAATTGCCAAAATTGAAAGAAGAAAAAAACAGTAAAACA ACAATTAATATTGAATAA Figure 2 (i) Is the nucleotide sequence above translatable into a complete protein? Justify your answer. (ii) Is the sequence above represented in the standard FASTA format? Explain your answer.
Below is the vector pUST, gene W, and the restriction cut sites. What are 3 cutting strategies for cloning gene W into pUST
CRISPR-cas9 is a powerful technique for gene editing. Please place the stages of a CRISPR-cas9 gene editing workflow in the correct order below options are on the right
Primers designing for epitope tagging: Design forward and reverse primers to amplify the following gene with 6×HIS-tag on the N-terminus of the protein. To be cleaved and inserted into the plasmid, add restriction sites for EcoRI and HindIII at 5' and 3'. ATGCTCTCCGCCCTCGCCCGGCCTGTCAGCGCTGCTCTCCGCCGCAGCTTCAGCACCTCAGCC CAGAACAATGCTAAAGTAGCTGTGCTAGGGGCCTCTGGAGGCATCGGGCAGCCACTTTCAC TTCTCCTGAAGAACAGCCCCTTGGTGAGCCGCCTGACCCTCTATGATATCGCGCACACACCC GGAGTGGCCGCAGATCTGAGCCACATCGAGACCAAAGCCGCTGTGAAAGGCTACCTCGGAC CTGAACAGCTGCCTGACTGCCTGAAAGGTTGTGATGTGTAA
You discover a new bacteria and are interested in mapping its genome with respect to 10 genes. You are able to infer time-of-entry maps for four Hfr strains, which are pictured below. What is the map of the bacterial genome based on these Hfr experiments? Hfr1 Ga E Ne Hfr2 Sq Lix Tf Ne Hfr3 U I D Ga Hfr4 D U Sq O Sq - Lix - Tf - Ne -E- M- Ga - D -1-U O Sq - Tf - Lix -U -E-D- Ne -D -M- Ga OD-1-U- Sq - Lix - Ga - E -M- Tf - Ne OD- Ne - Ga -E- M - Sq - I-U- Tf - Lix O Lix - Ga - E - Sq -1-U- M -D- Tf - Ne
Which RE can be used to distinguish bacterial strain 1 from strain 2 based on a sample DNA fragment from each strain (as shown below)? STRAIN 1 ~712 bp STRAIN 2 ~712 bp Restriction enzymes: O BglIII, Ndel, and Ncol O Ncol O Ndel OBgIIII CATATGCCATGGAGATCT GTATACGGTACCTCTAGA ~231 bp CACATGCCATGGAGATCT GTGTACGGTACCTCTAGA 231 bp BglIII Ncol AGATCT CCATGG TCTAGA GGTACC Nde1 CATATG GTATAC
what is the function and origin of this DNA sequence (use BLAST and NEBcutter)? ATGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGAAG ATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAACCCTGGA AAAAGCAGCAGTTGAAGCGTTTAAACTCGGTTACGAAGTGACCGATCCAGAAGAGCTGGAAGTTGAAGAT GGTGATATCGTGATTTGCTGCGACATCCTCAGCGAGTGCGCGTTGAATGCCGATCTGATCGATGCCCAGG TTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGA TCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAA
After cloning, they transformed and plate bacterial cells using their cloned plasmid. Onto what type of growth medium will they plate their cells in order to distinguish between bacterial cells that obtained the plasmid and those that did not?
Please help urgently
iii

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