...ATC ACC AAA AAC CGC AAC CTG CAC TCG... m allele 5’...ATC ACC AAA AAC TGC AAC CTG CAC TCG... A. Compare these two allele sequences and circle the nucleotides that are different. B. Do you think this change will cause a shift in the codon reading frame? Why or why not? C. Do you think that this change will cause an early stop in translation? Why or why not?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Task #1 Mc1r alleles: In Florida Gulf coast mice, there are two different alleles of the Melanocortin Receptor (Mcr1) gene. The sequence for both alleles is shown below. This is an internal segment of the coding (non-template) sequence of the Mcr1 gene. The reading frame is set and you do not need to find an AUG. M allele 5’...ATC ACC AAA AAC CGC AAC CTG CAC TCG... m allele 5’...ATC ACC AAA AAC TGC AAC CTG CAC TCG... A. Compare these two allele sequences and circle the nucleotides that are different.

B. Do you think this change will cause a shift in the codon reading frame? Why or why not?

C. Do you think that this change will cause an early stop in translation? Why or why not?

Task #2 Flow of information: A codon table is provided above. The 5’ codon nucleotide is in the left column, and second codon nucleotide is on top. The Mcr1 M and m allele sequences are shown again in the central dogma grids below with the reading frame designated. Fill in the grids. In the second column indicate the end polarity (5’ or 3’, N or C). For both alleles, determine the sequences of the DNA template strand, mRNA, tRNA and polypeptide.

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