Next, it's important to understand how the gene would be transcribed and translated. 1 agagtctcct cagacgccga gatgctggtc atggcgcccc gaaccgtcct cctgctgctc 61 tcggcggccc tggccctgac cgagacctgg gccggtgagt gcgggtcggg agggaaatgg 121 cctctgccgg gaggagcgag gggaccgсag gcgggggcgc atgacctcag gagccgcgcc 181 gggaggaggg tcgggcgggt ctcagcccct cctcaccccc aggctcccac tccatgtggt 241 atttctacac ctccgtatcc cggcccagcc gcggggagcc ccgcttcatc tcagtgggct 301 acqtggacga cacccagttc gtgaggttcg acagcgacgc cgcgagtccg agagaggagc 361 cgcgggcgcc gtggatagag caggaggggc cggagtattg ggaccggaac acacagatct 421 acaaggccca ggcacagact gaccgagaga gcctgcggaa cctgcgcttc tactacaacc 481 agagcgaggc cgttgcgtga ccccggcccg gggcgcaggt cacgactccc catcccccac 541 gtacggcccg ggtcgccccg agtctccggg tccgagatcc gcctccctga ggccgcggga a) coding region i) What is the length of the coding region (in nucleotides)? ii) Explain how you arrived at your answer for (i). iii) Which nucleotides are included in the coding region (e.g. nucleotides #20-50, inclusive)? b) polypeptide i) What will be the length of the resulting polypeptide (in amino acids)? Note that in some proteins the first methionine (encoded by the ATG start codon) is cleaved off, however the methionine is retained in this particular protein. ii) Explain how you calculated the number you provided for (i), above. c) C-terminal domain i) You're interested in expressing the C-terminal domain of the protein, which is encoded by amino acids number 45-56 (the C-terminal portion (domain) of a protein includes amino acids encoded by the nucleotides at the 3' end of the coding sequence). What is the range of nucleotides that corresponds to this region (e.g. nucleotides #20-50, inclusive)? Note that sometimes you may want to include the stop codon but in this case you do not.

Please asap

Use the graph to identify the most likely consensus sequences. Assume this is a prokaryoticcell, label Pribnow box

Figure out the mutation. You will need the codon table for this question. WT genomic sequence of a really small gene and matching complete amino acid sequence of its gene product. GGT ATG GGG ACT TTG AGG ATG ATA AGG CGT AAA TAA ATAT Met Gly Thr Leu Arg Met Ile Arg Arg Lys A mutant is found that has the following protein sequence: Met Gly Thr Leu Arg Gly. What is the likely mutation? between T14 and G15, insert T. G7 -> del. T5 -> del. G29, T30, A31 -> del between T14 and G15, insert AA. A31 -> T. C11 -> del

1. Use the prokaryotic gene DNA sequence below to answer the following questions: 1 11 21 ATGAAGCTAC 51 TTTGCCCAGG 101 ACATCTGACA 61 TTTGACAGTC TTCTATCGAA CAAGCATATA ATCAGCGATA 71 111 31 121 AGGTTTAACA ACATGTCAAG TGCTCCAAAG AAAAACCGAA 81 141 GGGAAAAAAT CCCCCCCCCC TTTTTTTTTTTTTTTTCCCG d. Where is the 3' UTR? Circle one. 12-72 35-72 131 109-146 41 a. Write the corresponding sequences, circle & label them in the sequence above: -35 consensus sequence: (label as -35) -10 consensus sequence (Pribnow box): Shine-Delgarno sequence in the corresponding mRNA: Start (initiation) codon in the corresponding mRNA: Stop (termination) codon in the corresponding mRNA: (label as PB) 91 c. What will be the nascent polypeptide sequence translated from this mRNA? (label as SD) (label as start) b. What region of this prokaryotic DNA sequence will be transcribed into mRNA? Circle one. 1-120 35-108 54-146 72-146 (label as stop) 121-146

The following sequence contains the first couple codons from the Moderna vaccine mRNA. Below it are versions of the same sequence, but each of these contain a mutation. Match these mutated sequences with the correct mutation type a b C d Original sequence AUG UUC GUG UUC CUG GUG CUG CUG CCC CUG GTG AGC AGC CAG UGC GUG AAC CUG AUG UUC GUG UUC CUG GUG CUG CUG CCC CUG GTG AGC AGC UAG UGC GUG AAC CUG AUG UUC GUG UCC CUG GUG CUG CUG CCC CUG GTG AGC AGC CAG UGC GUG AAC CUG AUG UUC GUG UUC CUG GUG CUG CUG CCA CUG GTG AGC AGC CAG UGC GUG AAC CUG AUG UUC GUG UUC CUG GUG CUG CUG CCC CUG GTG AGC AGC CAG UGC AGU GAA CCU 1. Synonymous 2. Nonsense 3. Non synonymous 4, Frameshift

Please answer asap and in short and content should not be palgarised please For each of the following RNA molecules: Indicate if it is Required or Not for translation in prokaryotes and, if it is required, what does it do? mRNA tRNA rRNA snRNA