Why transgene copy number is a key concern for transgenic studies? Describe an efficient method for the estimation of copy number.(Genetic engineering).
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QNo.3 Why transgene copy number is a key concern for transgenic studies? Describe an efficient method for the estimation of copy number.(Genetic engineering).
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- o How do SNPs arise?describe one example of an SNP cause of a geneticdisease describe one example of an SNP that resulted in anadvantage to the organism.. Propose a mechanism by which a type II topoisomerase could use the energy of ATP hydrolysis to scan a large DNA molecule and, thereby, to direct that the enzyme will catalyze largely “disentan- gling" reactions (decatenation and unknotting).Q.8. What is recombination? Mention its applications with reference to genetic engineering.
- Besides using A. tumefaciencs in the generation of transgenic plants, elaborate on the THREE (3) methods to introduce genc of interest into a plant cell.Please make this as SIMPLE as possible Question: regarding to horizontal gene transfer please only discuss what cells are still alive or dead between transformation, trasnduction, and conjugation Be specific when discussing the donor versus recipient cell, and if the donor and recipient cells are still alive after each horizontal gene transfer event is completeDescribe the rationale behind the electrophoretic mobility shift assay.
- Q 1. Download and read the attached review article https://www.ncbi.nlm.nih.gov/pmc/articles/PMC342244/pdf/nar00470-0155.pdf titled: MspI, an isoschizomer of HpalI which cleaves both unmethylated and methylated Hpall sites and answer the following question: Restriction enzymes have many applications from gene cloning to gene activity and mapping. Use the two above mentioned restriction enzymes to study transcriptional silencing (consider Southern blot method for your example)Need help, please. 1) Which primer could be used as the forward primer, for synthesizing DNA from left to right in the above diagram? Enter the number of the primer here: 2) Which primer could be used as the reverse primer, for synthesizing DNA from right to left in the above diagram? Enter the number of the primer here: 3) Will these primers be part of the final PCR product? a.No, neither primer will be incorporated into the PCR product. b.The forward primer but not the reverse primer will be incorporated into the PCR product. c.The reverse primer but not the forward primer will be incorporated into the PCR product. d.Yes, both primers will be incorporated into the PCR product.10:25 Name_ Bio320 What is lignin and its role in plants? Why can reducing the amount of lignin in trees enhance the efficiency of cellulostic-ethanol production? Why can't trees be genetically engineered without any lignin at all? 5Gº Why are transgenic approaches rather than traditional breeding being used to create trees with altered levels of lignin? Where can you find the only company in the USA that makes transgenic trees that are found in forests? Go to this company's website. What type of endangered tree are they trying to survive? AA moodle22-23.coastal.edu
- Besides using vector-based methods to transform crop plants, elaborate on THREE (3) other methods to introduce gene of interest into plant cells for the production of transgenic crops.True or False: 1. The 6X HIS tag is required for a eukaryotic protein to be expressed in E. coli 2. BAC vectors are an appropriate choice for cloning cDNAs 3. Cluster analysis of micro-array data groups together mRNAs of similar sequence 4. In genomic DNA, on average EcoR1 restriction sites are more common than Mse1 restriction sites 5. Tags such as HA that are fused to proteins always compromise their function.What are site-recombinases? Describe in detail how cre- recombinase can be used to decipher the roles of specific genes and proteins in complex multicellular organisms? Explain how the cre-lox system can be used to examine the role of a particular gene in a specific type of tissue?