MATERIALS AND METHODS Molecular Characterization of Respiratory Allergy, Allergic Asthma, and Non-Allergic Asthma Firstly, the molecular characterization of the three pathophysiological processes of interest (respiratory allergy, allergic asthma, and nonallergic asthma) was performed using the Therapeutic Performance Mapping System (TPMS) technology (Anaxomics Biotech, Barcelona, Catalonia, Spain) (31). Briefly, systems biology generates models that are able to reproduce the behavior of a disease in a patient, thus identifying the key genes, proteins, or metabolites in the development of the disease. A dictionary has been created to translate clinical and medical terms into molecular biology data, effectively linking the molecular and the clinical words. This dictionary, called the Biological Effectors Database (BED), relates biological processes (adverse events of drugs, drug indications, diseases, etc.) with the proteins most closely associated with them. Thus, the dictionary acts as a translator of clinical phenotypes into terms comprehensible for protein networks, and conversely allows for the translation of molecular measures toward clinical outcomes. The BED is structured hierarchically, where the biggest level is the entire disease, which is divided into different pathophysiological molecular motifs, which in turn contain the proteins involved in the development of the disease. The motifs are classified into two levels depending on their respective implication, i.e. causal motifs, which are directly related to the onset or pathophysiology of the condition, and symptomatic (manifestative) motifs, which are a consequence of the disease. In the present study, respiratory allergy, allergic asthma, and non-allergic asthma have been characterized at the molecular level. Therefore, the analysis of high throughput data by means of TPMS allows for identification of those proteins closely associated with the disease of interest and can provide a mechanistic rationale for their involvement. The effector proteins of the manifestative and causal molecular motifs of these three diseases have been identified through bibliographic review and curate data.
Transcribed Image Text:MATERIALS AND METHODS
Molecular Characterization of Respiratory
Allergy, Allergic Asthma, and Non-Allergic
Asthma
Firstly, the molecular characterization of the three pathophysiological
processes of interest (respiratory allergy, allergic asthma, and non-
allergic asthma) was performed using the Therapeutic Performance
Mapping System (TPMS) technology (Anaxomics Biotech,
Barcelona, Catalonia, Spain) (31). Briefly, systems biology generates
models that are able to reproduce the behavior of a discase in a
patient, thus identifying the key genes, proteins, or metabolites in the
development of the discase. A dictionary has been created to translate
dinical and medical terms into molecular biology data, effectively
linking the molecular and the dinical words. This dictionary, called
the Biological Effectors Database (BED), relates biological processes
(adverse events of drugs, drug indications, diseases, etc.) with the
proteins most closely associated with them. Thus, the dictionary acts
as a translator of clinical phenotypes into terms comprehensible for
protein networks, and conversely allows for the translation of
molecular measures toward clinical outcomes. The BED is
April 2021 | Volume 12 | Article 640791
des-Jimeno et al.
Asthma Biomarkers by Syste
structured hierarchically, where the biggest level is the entire disease,
which is divided into different pathophysiological molecular motifs,
which in turn contain the proteins involved in the development of the
disease. The motifs are classified into two levels depending on their
respective implication, ie. causal motifs, which are directly related to
the onset or pathophysiology of the condition, and symptomatic
(manifestative) motifs, which are a consequence of the disease.
In the present study, respiratory allergy, allergic asthma, and
non-allergic asthma have been characterized at the molecular level.
Therefore, the analysis of high throughput data by means of TPMS
allows for identification of those proteins closcly associated with
the disease of interest and can provide a mechanistic rationale for
their involvement. The effector proteins of the manifestative and
causal molecular motifs of these three diseases have been identified
through bibliographic review and curate data. Figure 1
summarizes the workflow used for this study.
Definition Definition Observable physical traits or characteristics of an organism, which are determined by its genetic makeup (genotype) as well as environmental factors. These traits can include anything from eye color or height to behavior or disease susceptibility. Understanding an organism's phenotype is important in fields such as genetics, medicine, and ecology.
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