Task #1 Mc1r alleles: In Florida Gulf coast mice, there are two different alleles of the Melanocortin Receptor (Mcr1) gene. The sequence for both alleles is shown below. This is an internal segment of the coding (non-template) sequence of the Mcr1 gene. The reading frame is set and you do not need to find an AUG. M allele m allele 5'...ATC ACC AAA AAC CGC AAC CTG CAC TCG... 5'...ATC ACC AAA AAC TGC AAC CTG CAC TCG... A. Compare these two allele sequences and circle the nucleotides that are different. B. Do you think this change will cause mRNA Codon Table Second base a shift in the codon reading frame? Why or why not? C. Do you think that this change will cause an early stop in translation? Why or why not? First base U U Phe Phe Leu Leu C Leu Leu Leu Leu A lle lle lle Met G Val Val Val Val C Ser Ser Ser Ser Pro A G Cys Tyr Cys STOP Trp STOP STOP A His Arg Pro His Pro Gin Pro Gin Thr Asn Thr Asn Thr Lys Thr Lys Ala Ala Ala Ala Arg Arg Arg Ser Ser Arg Arg Gly Asp Asp Glu Gly Glu Gly UUAGUUAGSUAGUUAG Gly C C А C А C А Third base
Task #1 Mc1r alleles: In Florida Gulf coast mice, there are two different alleles of the Melanocortin Receptor (Mcr1) gene. The sequence for both alleles is shown below. This is an internal segment of the coding (non-template) sequence of the Mcr1 gene. The reading frame is set and you do not need to find an AUG. M allele m allele 5'...ATC ACC AAA AAC CGC AAC CTG CAC TCG... 5'...ATC ACC AAA AAC TGC AAC CTG CAC TCG... A. Compare these two allele sequences and circle the nucleotides that are different. B. Do you think this change will cause mRNA Codon Table Second base a shift in the codon reading frame? Why or why not? C. Do you think that this change will cause an early stop in translation? Why or why not? First base U U Phe Phe Leu Leu C Leu Leu Leu Leu A lle lle lle Met G Val Val Val Val C Ser Ser Ser Ser Pro A G Cys Tyr Cys STOP Trp STOP STOP A His Arg Pro His Pro Gin Pro Gin Thr Asn Thr Asn Thr Lys Thr Lys Ala Ala Ala Ala Arg Arg Arg Ser Ser Arg Arg Gly Asp Asp Glu Gly Glu Gly UUAGUUAGSUAGUUAG Gly C C А C А C А Third base
Biology (MindTap Course List)
11th Edition
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Chapter15: Dna Technology And Genomics
Section15.3: Tools For Studying Dna
Problem 11LO: Discuss how qPCR, DNA microarrays (DNA chips), and RNA-seq analysis are used to compare levels of...
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