Task #1 Mc1r alleles: In Florida Gulf coast mice, there are two different alleles of the Melanocortin Receptor (Mcr1) gene. The sequence for both alleles is shown below. This is an internal segment of the coding (non-template) sequence of the Mcr1 gene. The reading frame is set and you do not need to find an AUG. M allele m allele 5'...ATC ACC AAA AAC CGC AAC CTG CAC TCG... 5'...ATC ACC AAA AAC TGC AAC CTG CAC TCG... A. Compare these two allele sequences and circle the nucleotides that are different. B. Do you think this change will cause mRNA Codon Table Second base a shift in the codon reading frame? Why or why not? C. Do you think that this change will cause an early stop in translation? Why or why not? First base U U Phe Phe Leu Leu C Leu Leu Leu Leu A lle lle lle Met G Val Val Val Val C Ser Ser Ser Ser Pro A G Cys Tyr Cys STOP Trp STOP STOP A His Arg Pro His Pro Gin Pro Gin Thr Asn Thr Asn Thr Lys Thr Lys Ala Ala Ala Ala Arg Arg Arg Ser Ser Arg Arg Gly Asp Asp Glu Gly Glu Gly UUAGUUAGSUAGUUAG Gly C C А C А C А Third base

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ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
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Chapter15: Dna Technology And Genomics
Section15.3: Tools For Studying Dna
Problem 11LO: Discuss how qPCR, DNA microarrays (DNA chips), and RNA-seq analysis are used to compare levels of...
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Task #1 Mc1r alleles: In Florida Gulf coast mice, there are two different alleles of the
Melanocortin Receptor (Mcr1) gene. The sequence for both alleles is shown below. This
is an internal segment of the coding (non-template) sequence of the Mcr1 gene. The
reading frame is set and you do not need to find an AUG.
M allele
m allele
5'...ATC ACC AAA AAC CGC AAC CTG CAC TCG...
5'...ATC ACC AAA AAC TGC AAC CTG CAC TCG...
A. Compare these two allele sequences and circle the nucleotides that are different.
B. Do you think this change will cause
mRNA Codon Table
Second base
a shift in the codon reading frame?
Why or why not?
C. Do you think that this change will
cause an early stop in translation?
Why or why not?
First base
U
U Phe
Phe
Leu
Leu
C Leu
Leu
Leu
Leu
A lle
lle
lle
Met
G Val
Val
Val
Val
C
Ser
Ser
Ser
Ser
Pro
A
G
Cys
Tyr Cys
STOP Trp
STOP STOP A
His Arg
Pro
His
Pro Gin
Pro Gin
Thr
Asn
Thr
Asn
Thr
Lys
Thr
Lys
Ala
Ala
Ala
Ala
Arg
Arg
Arg
Ser
Ser
Arg
Arg
Gly
Asp
Asp
Glu Gly
Glu
Gly
UUAGUUAGSUAGUUAG
Gly
C
C
А
C
А
C
А
Third base
Transcribed Image Text:Task #1 Mc1r alleles: In Florida Gulf coast mice, there are two different alleles of the Melanocortin Receptor (Mcr1) gene. The sequence for both alleles is shown below. This is an internal segment of the coding (non-template) sequence of the Mcr1 gene. The reading frame is set and you do not need to find an AUG. M allele m allele 5'...ATC ACC AAA AAC CGC AAC CTG CAC TCG... 5'...ATC ACC AAA AAC TGC AAC CTG CAC TCG... A. Compare these two allele sequences and circle the nucleotides that are different. B. Do you think this change will cause mRNA Codon Table Second base a shift in the codon reading frame? Why or why not? C. Do you think that this change will cause an early stop in translation? Why or why not? First base U U Phe Phe Leu Leu C Leu Leu Leu Leu A lle lle lle Met G Val Val Val Val C Ser Ser Ser Ser Pro A G Cys Tyr Cys STOP Trp STOP STOP A His Arg Pro His Pro Gin Pro Gin Thr Asn Thr Asn Thr Lys Thr Lys Ala Ala Ala Ala Arg Arg Arg Ser Ser Arg Arg Gly Asp Asp Glu Gly Glu Gly UUAGUUAGSUAGUUAG Gly C C А C А C А Third base
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