Restriction Digestion of E

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Dear All, Question for review 1. Discuss the following terminologies a) Mesosomes b) Plasmids c) LPS d) O antigen e) H antigen f) Capsule g) flagella h) pili i) endospore 2. Basic properties of nucleoid 3. The structure and chemical components of bacterial cell walls

21:14 +4G KB/S LTED 41 96 09.00 Vo Microsoft Word - Assignment 02.docx Assignment 02 1. Groups of alleles associated with the lactose operon are as follows (in order of dominance for each allelic series): repressor, IS (superrepressor), I *(inducible), and I "(constitutive); operator, OC (constitutive, cis dominant) and 0+ (inducible, cis- dominant); structural, Z and Y. (a) Which of the following genotypes will produce B-galactosidase and B-galactoside permease if lactose is present: (1) 1*0* Z* Y*, (2) 1 *OCZ+ Y+, (3) OCZ+ Y+, (4) IS O+ Z+ Y+, and (5) 10+ Z+Y+? (b) Which of the above genotypes will produce ß-galactosidase and ß-galactoside permease if lactose is absent? Why? 2. By what mechanism does the presence of tryptophan in the medium in which E. coli cells are growing result in premature termination or attenuation of transcription of the trp operon? 3. Suppose that you used site-specific mutagenesis to modify the trpL sequence such that the two UGG Trp codons at positions…

pMBBS plasmid was subjected to complete digestion by different combinations of three restrictive enzymes (EcoRI, BamHI, and XhoI), and the results analyzed  on an agarose gel, Figure 1. Using the data, respond to the question below Draw the actual map of the pMBBS plasmid, following the style of the sample map shown in Figure Make sure you show the following information: (1) relative locations of the RE sites; (2) size in base pairs of the segments of DNA between sites. (The map need not be drawn to scale.)

Theoretical Data Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1 Chloroform-treated lysate 1ml Dilution 10-3 10-4 10-5 0 0 0 0 0 9ml 9ml 9ml 10-1 10-² 10-3 Empty sterile tubes Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix 0.1 ml 9ml 9ml 104 10.5 // 0.1 ml 0.1 ml +0.5 ml E. coli ↓↓↓↓↓↓ Incubate O/N at 35°C, observe for plaques Molten soft agar overlay B. Quantification of the Infective E. coli other Bacteriophage Particles in the Raw Sewage Sample Volume of Stock Plated (in ml) Bottom agar No. of Plaque-Forming Units (pfu) Ave. A B 254 265 132 110 11 23

Biol370 - Assignment #2 – Spring 2020: Replication - Transcription - Translation Due 3/10/2020 (Before the beginning of the class) Name ID Instruction: 1. Print this document (US-letter only) Fill the table below with the appropriate color (see coloring rule) 3. Complete and annotate the drawing in attachment (see page #2). Make sure all the listed terms are present in your final drawing! (graded using 5 randomly selected terms 2. This type of drawing makes only sense for Eukaryotes/Prokaryotes (circle one) because: or The annotation must follow the coloring rule : DNA in BLACK. RNA in RED. Proteins and AAs in GREEN. Complex structures with some DNA and/or RNA and/or Proteins in BLUE. Color Color Color DNA DNA pol III Stop codon RNA RNA polymerase 5'-UTR Peptide Helicase 3'-UTR Leading strand Topoisomerase CDS Lagging strand Ligase RBS Coding strand Primer Ribosome SSU Template strand -10/-35 Box Ribosome LSU 5'/3' ends (all) Transcription start AA-TRNA N-/C- terminal ends…

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Question:- There is some recent research on the use of bacteriophages to inhibit pathogenic bacteria as alternative to antibiotics, for example, in poultry. Speculate what are the potential advantages and weaknesses of such phage therapy vs standard antibiotic treatment.

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SARS-CoV-2 has 30,000 nucleotides? How long is this compared to bacterial genomes and to human genomes.

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Prepare a simple flowchart of the experimental procedures for Bacteria Preparation and Transformation the notes are attached below