EcoRI BamHI Xhol III +11 1+1 11+ 1++ +1+ ++ 1 DNA size ladder *The same total amount of DNA was loaded in each lane. 3000 bp 2500 bp 2000 bp 1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp VC 100bp Plus DNA ladder from Vivantis Technologies

What would happen to a bacteria that lost a plasmid for antibiotic resistance?  Would it still be able to grow in normal growth conditions?  Explain.

Hi, I am working on qPCR quantification for specific bacteria. However, when the CT value achieve greater than 30, it always shown high CT value differences in my duplication no matter I repeat the same set of experiment for those samples with low quality. My question is, what makes this happening? The other question is, how many CT value differences is acceptable for duplication?

The modifiedplasmid is reintroduced back into Rhizobium(step 4) and the genetically transformed bacteria are then selected based on the amp and lacZgenes present within the plasmid. The plasmid may or may not integrate the BBW resistancegene. The treated bacteria may or may not take up the modified plasmid.   a) Complete the table below with a yes or no in each space stating whether you would expect these bacteria to grow or not. Type of treated bacteria culture plate(no amp) Culture plate treated with ampicillin   Plasmid not taken up     Plasmid taken up (WithoutBBW resistancegene)     Plasmid taken up (WithBBW resistancegene)     B) Outline how the genetically transformed bacteria containing the BBW resistance gene can beselected based on the amp and lacZgenes present within the plasmid.