There is some recent research on the use of bacteriophages to inhibit pathogenic bacteria as alternative to antibiotics, for example, in poultry. Speculate what are the potential advantages and weaknesses of such phage therapy vs standard antibiotic treatment.
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Question:-
There is some recent research on the use of bacteriophages to inhibit pathogenic bacteria as alternative to antibiotics, for example, in poultry. Speculate what are the potential advantages and weaknesses of such phage therapy vs standard antibiotic treatment.
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- Discuss some advantages and disadvantages of bacteriophage therapyin treating bacterial infections.About the technique of phage display: MOLECULAR BIOLOGY_advanced The Escherichia coli cell infected by the phage codifies for the optimized ligand when the phage DNA integrates in the DNA of the bacteria. Phages are selected if they express on their surface the optimized ligand. One selects Escherichia coli cells that are resistant to the phage infection. More than one optimized ligand can be selected during the panning procedure. The ligand to be selected on the surface of the phage is non-covalently linked to one of the surface proteins.Match each of the descriptions in Column A with the correct panel listed in Column B. Description Panel Resistant bacteria share resistance genes with each other Resistant bacteria survive and reproduce Treatment with methicillin kills nonresistant bacteria Some bacteria carry a mutation that provided resistance to methicillin Predict the possible outcome(s) if the antibiotic tetracycline was applied after panel 4. Check all that apply. O The tetracycline will act as a selective pressure, causing bacteria to mutate in order to survive. O Since the population is resistant to methicillin and not tetracycline, none of the bacteria will survive. O Treatment with an antibiotic will not affect the overall survival of the population. Any bacterium that has a mutation for tetracycline resistance would be able to survive.
- a. Explain what is happening the figure below. What important implication originated from The Hershey-Chase Experiment. (A) 32p-labeled phage DNA (B) 355-labeled phage proteins unlabeled 355-labeled empty phages empty phages infected bacteria unlabeled infected bacteria labeled with 3²pQuestion: Gene therapy: a) what disease is it used for? b) what genetic defect causes the disease? c) what and how viral vector is used AND/OR CRISPR-Cas is used? d) what is in the horizen re. development of new virus-based gene delivery vesicle?Part 1. Gene Cloning 1) How is a gene inserted into a plasmid cloning vector? Be specific, but be brief. No more than 2 (non-run-on) sentences please, and do not exceed this space. 2) A geneticist has transformed a vector with an ampicillin resistance gene into E. coli cells and plated those cells on media containing ampicillin. She comes back 24 hours later and sees only blue colonies on the media. a) How does she know that the cells growing on the media were successfully transformed with the vector? b) What does it mean that the colonies are blue?
- Which of the following apply to the development of urogenital tract Candida albicans infections? Disruption of population dynamics allowing the RB form of Chlamydia to cause tissue damage Loss of Lactobacillus populations leading to an increase in the local pH Loss of Gardnerella vaginalis populations leading to a lose of Clue cells Loss of the anaerobic environment that promotes the outgrowth of Trichomonas vaginalis None of the answers applyPapillomavirus QUESTION 39 ... In 2007, a Japanese scientist determined that pluripotent cells could be induced by. Introducing 4 regulatory genes into a fully differentiated cell Culturing stem cells in nutrient rich agar under different conditions Using a retrovirus to turn stem cells into progenitor cells Replacing non-reproducing stem cells with reproducing ones None of the above QUESTION 40 Fapsids are made of,,How important is RNA extraction in the recent pandemic? Explain in maximum of 5 sentences how it is being applied and what is the procedure behind to get a fast, rapid and high quality RNA sample. Include references.
- Describe what will happen to bacteria with dysfunctional restriction modification system providing that restrictase (first scenario) or methylase (second scenario) is not functional when it gets attacked by phage or by another bacterial species. T: BI1. Precise words:Find the nonspecific terms in the following sentences. Replace the nonspecific choices with more preciseterms or phrases (It is not necessary to change the sentence structure).(i) All OVE mutants showed enhanced iP concentrations.(ii) Plants were kept in the cold overnight.(iii) To provide proof of concept for our hypothesis, we studied a virus in its host cell.(iv) The present paper reports on continuing experiments that were performed to clarify thissurprising effect.(v) The first transition state is a little lower in energy than the second transition state. 2. Simple words:Improve the word choice in the following examples by replacing the underlined terms or phrases withsimpler word choices (do not change the sentence structure).(i) These data substantiate our hypothesis.(ii) The difference in our results compared to those of Reuter et al. (1995) can be accounted forby the fact that different conditions were used.(iii) For the purpose of discussing cell migration we…To describe: The significance of the differences between the ribosomes of eukaryotic and prokaryotic organisms in terms of antibiotic therapy.