Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Chapter 9.3, Problem 3CC
Summary Introduction
To compare: The multi-enzyme complex involved in the conversion of pyruvate to acetyl CoA with the multienzyme complex that catalyzes Step 4 of the Citric acid cycle.
Introduction:
The citric acid cycle is a cyclic
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Kinetic Parameters of Enzyme-Catalyzed Reactions
TABLE 12-1 The Values of KM, Keat, and Keat/KM for Some Enzymes and Substrates
Enzyme
Substrate
KM (M)
9.5 x 10-5
1.2 x 10-²
2.6 x 10-2
2.5 x 10-2
4.4 x 10-1
8.8 x 10-2
6.6 x 10-4
Acetylcholinesterase
Carbonic anhydrase
Catalase
Chymotrypsin
Fumarase
Urease
Acetylcholine
CO₂
HCO₁
H₂O₂
N-Acetylglycine ethyl ester
N-Acetylvaline ethyl ester
N-Acetyltyrosine ethyl ester
Fumarate
Malate
Urea
5.0 x 10-6
2.5 x 10-5
2.5 x 10-2
Keat (S-¹)
1.4 x 104
1.0 × 106
4.0 × 105
1.0 X 107
5.1 x 10-2
1.7 × 10-1
1.9 X 10²
8.0 x 10²
9.0 × 10²
1.0 X 104
Keat/KM (M¹s¹)
1.5 × 108
8.3 x 107
1.5 x 107
4.0 X 108
1.2 x 10-1
1.9
2.9 × 105
1.6 × 108
3.6 X 107
4.0 X 105
Which enzyme is the most catalytically efficient?
Which substrate does chymotrypsin bind to most tightly (assume k_₁ >> K₂)?
Is fumarate or malate a better substrate of fumarase?
Is it possible to have a kcat/KM of greater than 1 x 10⁹ M-¹ s-¹? Why or why not?
What is the catalytic efficiency of Catalase ?
Table. The values of KM and kcat for some Enzymes and Substrates
Enzyme
Carbonic anhydrase
Substrate
CO2
HCO3
KM (M)
1.2 x 10-2
2.6 x 10-2
Kcat (s-1)
1.0 x 106
4.0 x 105
Catalase
H2O2
2.5 x 10-2
1.0 x 107
Urease
Urea
2.5 x 10-2
4.0 x 105
O A. 4 x 108 M-s-1
O B. 4 x 108 M-1.s-1
OC25x 10-9 M-s1
D. 2.5 x 102 M-1.s-1
OE 1.0 x 107 s1
Chapter 9 Solutions
Campbell Biology
Ch. 9.1 - Compare and contrast aerobic and anaerobic...Ch. 9.1 - WHAT IF? If the following redox reaction...Ch. 9.2 - Prob. 1CCCh. 9.3 - Prob. 1CCCh. 9.3 - What process in your cells produce the CO2 that...Ch. 9.3 - Prob. 3CCCh. 9.4 - Prob. 1CCCh. 9.4 - WHAT IF? In the absence of O2 as in question 1,...Ch. 9.4 - MAKE CONNECTIONS Membranes must be fluid to...Ch. 9.5 - Consider the NADH formed during glycolysis. What...
Ch. 9.5 - WHAT IF? A glucose-fed yeast cell is moved from...Ch. 9.6 - MAKE CONNECTIONS Compare the structure of a fat...Ch. 9.6 - Prob. 2CCCh. 9.6 - Prob. 3CCCh. 9.6 - Prob. 4CCCh. 9 - Describe the difference between the two processes...Ch. 9 - Which reactions in glycolysis are the source of...Ch. 9 - What molecular products indicate the complete...Ch. 9 - Briefly explain the mechanism by which ATP...Ch. 9 - Prob. 9.5CRCh. 9 - Prob. 9.6CRCh. 9 - Level 1: Knowledge/Comprehension 1. The immediate...Ch. 9 - Prob. 2TYUCh. 9 - Prob. 3TYUCh. 9 - Prob. 4TYUCh. 9 - What is the oxidizing agent in the following...Ch. 9 - When electrons flow along the electron transport...Ch. 9 - Prob. 7TYUCh. 9 - Prob. 8TYUCh. 9 - Prob. 9TYUCh. 9 - Prob. 10TYUCh. 9 - INTERPRET THE DATA Phosphofructokinase is an...Ch. 9 - DRAW IT The graph here shows the pH difference...Ch. 9 - EVOLUTION CONNECTION AIP synthases are found in...Ch. 9 - SCIENTIFIC INQUIRY In the 1930s, some physicians...Ch. 9 - WRITE ABOUT A THEME: ORGANIZATION In a short essay...Ch. 9 - SYNTHESIZE YOUR KNOWLEDGE Coenzyme Q (CoQ) is sold...
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