Campbell Biology
Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Chapter 9, Problem 8TYU
Summary Introduction

To analyze: ATP that is considered to be an allosteric regulator of phosphofructokinase inhibits or stimulates the activity of this enzyme.

Introduction:

Phosphofructokinase is the most important regulatory enzyme in glycolysis, converting fructose-6 phosphate to fructose-1,6 bisphosphate. Its regulation is allosteric, with adenosine triphosphate (ATP) acting as the allosteric regulator. This enzyme plays a key role in the energy investment step of glycolysis.

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Back in 1962, Gerhart and Pardee developed a model for the regulation of the activity of the ATCase enzyme by CTP and ATP, using the pathway given in Figure 1. Describe that model, using information presented here as well as what you have learned about allosteric enzymes. Be sure to include a sentence explaining the physiological significance of your model.
. In another key reaction in glycolysis, dihydroxyacetone phosphate (DHAP) is isomerized into glyceraldehyde-3-phosphate (GAP) Because ∆G°′ is positive, the equilibrium lies to the left. (a) Calculate the equilibrium constant and the equilibrium fraction of GAP from the above, at 37 °C. (b) In the cell, depletion of GAP makes the reaction proceed. What will ∆G be if the concentration of GAP is always kept at 1/100 of the concentration of DHAP?
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