Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 6.2, Problem 5CC
Retrieve, Infer, Apply
5. All four
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AGAROSE GEL ELECTROPHORESIS PRELAB
ANSWER ALL QUESTIONS
1. Type of nucleotide is one of the factors that influence electrophoretic mobility.
a) True
b) False
2. Electrophoresis is used for DNA separation and not proteins.
a) True
b) False
3. DNA Polymerase is an enzyme to cut DNA into fragments for electrophoresis.
a)True
b)False
4. Electrophoresis apparatus consists of Gel buffer, chamber and DNA separator.
a) True
b) False
5. During electrophoresis, DNA fragments collect at the Cathode.
a) True
b) False
EcoRI
1.1kbp
1.5 kbp
Hindll
Hindll
0.15 kbp
amp
0.25 kbp
EcoRI
If you were to digest this plasmid with HindllI, how many fragments would be visible using gel electrophoresis?
O 1
O 4
Chapter 6 Solutions
Prescott's Microbiology
Ch. 6.2 - MICRO INQUIRY Which capsids are icosahedral? Which...Ch. 6.2 - Prob. 2MICh. 6.2 - MICRO INQUIRY Why is T4 said to have binal...Ch. 6.2 - Retrieve, Infer, Apply 1. How are viruses similar...Ch. 6.2 - Retrieve, Infer, Apply 2. What is the difference...Ch. 6.2 - Retrieve, Infer, Apply 3. Compare the structure of...Ch. 6.2 - Prob. 4CCCh. 6.2 - Retrieve, Infer, Apply 5. All four nucleic acid...Ch. 6.3 - MICRO INQUIRY Which of these mechanisms involves...Ch. 6.3 - MICRO INQUIRY Why do the empty capsids remain...
Ch. 6.3 - Explain why the receptors that viruses have...Ch. 6.3 - What probably plays the most important role in...Ch. 6.3 - How do you think the complexity of the viral...Ch. 6.3 - In general, DNA viruses can be much more dependent...Ch. 6.3 - Consider the origin of viral envelopes and suggest...Ch. 6.3 - Why are the proteins involved in virion assembly...Ch. 6.4 - Why is a lysogen considered a new or different...Ch. 6.4 - Define the terms lysogeny, temperate phage,...Ch. 6.4 - What advantages might a phage gain by being...Ch. 6.4 - Describe lysogenic conversion and its...Ch. 6.4 - How does a latent infection differ from a chronic...Ch. 6.4 - What is a cytocidal infection? What is a...Ch. 6.4 - Define the following terms: tumor, neoplasia,...Ch. 6.4 - Distinguish the mechanism by which dsDNA viruses...Ch. 6.5 - Prob. 1MICh. 6.5 - Prob. 1CCCh. 6.5 - Given that viruses must be cultivated to make...Ch. 6.7 - What are viroids and why are they of great...Ch. 6.7 - How does a viroid differ from a virus? From a...Ch. 6.7 - What is a prion? In what way does a prion differ...Ch. 6.7 - Prob. 4CCCh. 6 - Prob. 1RCCh. 6 - Prob. 2RCCh. 6 - Many classification schemes are used to identify...Ch. 6 - The origin and evolution of viruses is...Ch. 6 - Consider the separate stages of an animal virus...
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