Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Textbook Question
Chapter 6.2, Problem 2CC
Retrieve, Infer, Apply
2. What is the difference between a nucleocapsid and a capsid?
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Explain the two-dimensional gel electrophoresis (2DGE) ?
A. Basic mechanism of 2d gel electrophoresis techniqes?
B. How do we predict the protein structure from DNA sequence?
AGAROSE GEL ELECTROPHORESIS PRELAB
ANSWER ALL QUESTIONS
1. Type of nucleotide is one of the factors that influence electrophoretic mobility.
a) True
b) False
2. Electrophoresis is used for DNA separation and not proteins.
a) True
b) False
3. DNA Polymerase is an enzyme to cut DNA into fragments for electrophoresis.
a)True
b)False
4. Electrophoresis apparatus consists of Gel buffer, chamber and DNA separator.
a) True
b) False
5. During electrophoresis, DNA fragments collect at the Cathode.
a) True
b) False
Chapter 6 Solutions
Prescott's Microbiology
Ch. 6.2 - MICRO INQUIRY Which capsids are icosahedral? Which...Ch. 6.2 - Prob. 2MICh. 6.2 - MICRO INQUIRY Why is T4 said to have binal...Ch. 6.2 - Retrieve, Infer, Apply 1. How are viruses similar...Ch. 6.2 - Retrieve, Infer, Apply 2. What is the difference...Ch. 6.2 - Retrieve, Infer, Apply 3. Compare the structure of...Ch. 6.2 - Prob. 4CCCh. 6.2 - Retrieve, Infer, Apply 5. All four nucleic acid...Ch. 6.3 - MICRO INQUIRY Which of these mechanisms involves...Ch. 6.3 - MICRO INQUIRY Why do the empty capsids remain...
Ch. 6.3 - Explain why the receptors that viruses have...Ch. 6.3 - What probably plays the most important role in...Ch. 6.3 - How do you think the complexity of the viral...Ch. 6.3 - In general, DNA viruses can be much more dependent...Ch. 6.3 - Consider the origin of viral envelopes and suggest...Ch. 6.3 - Why are the proteins involved in virion assembly...Ch. 6.4 - Why is a lysogen considered a new or different...Ch. 6.4 - Define the terms lysogeny, temperate phage,...Ch. 6.4 - What advantages might a phage gain by being...Ch. 6.4 - Describe lysogenic conversion and its...Ch. 6.4 - How does a latent infection differ from a chronic...Ch. 6.4 - What is a cytocidal infection? What is a...Ch. 6.4 - Define the following terms: tumor, neoplasia,...Ch. 6.4 - Distinguish the mechanism by which dsDNA viruses...Ch. 6.5 - Prob. 1MICh. 6.5 - Prob. 1CCCh. 6.5 - Given that viruses must be cultivated to make...Ch. 6.7 - What are viroids and why are they of great...Ch. 6.7 - How does a viroid differ from a virus? From a...Ch. 6.7 - What is a prion? In what way does a prion differ...Ch. 6.7 - Prob. 4CCCh. 6 - Prob. 1RCCh. 6 - Prob. 2RCCh. 6 - Many classification schemes are used to identify...Ch. 6 - The origin and evolution of viruses is...Ch. 6 - Consider the separate stages of an animal virus...
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- what are two differences between agarose gel electrophoresis and SDS PAGE?arrow_forwardpls do not copy paste Explain the steps of preparing a cell culture stock from a T25 flask.arrow_forwardMicrob briefly describe 3 possible effects that antibiotics have on bacteria (ie- 3 things antibiotics can do to the bacterial cell). Indicate whether each effect is bacteriocidal or bacteriostatic.arrow_forward
- Besides agarosc gel electrophoresis, there is also SDS-PAGE for separation of macromolccules. Differentiate both these methods.arrow_forwardWhat makes platinum replica EM more desirable than conventional SEM and TEM (address both, specifically) for imaging cytoskeleton?arrow_forwardCan two-dimensional gel electrophoresis be used as a purificationtechnique? Explain.arrow_forward
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- Explain two-dimensional gel electrophoresis in detail.arrow_forwardWhy is a microtome an indispensable tool in a Microtechnique laboratory?arrow_forwardClinical application: A 44-year-old man with HIV is receiving antibiotics through a intravenous catheter. The antibiotics are to help treat a kidney infection. The patient develops a fever. Subsequent cultures from the patient's blood, the needle tip, and from the insertion site all show growth of an organism with large oval-shaped cells. The cells reproduce by budding. (a) What is your guess about the identity of the pathogen? (b) How do you think the antibiotics may have contributed to this outcome? (c) What do you think the portal of entry was for this pathogen?arrow_forward
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