Biology
Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 6.2, Problem 2CC
Summary Introduction

To determine: The reaction which would possess higher velocity among the two reactions catalyzed by enzyme A and enzyme B respectively.

Introduction: Vmax is the maximum value of velocity which is attained by the enzyme and after this value, the enzyme activity decreases.Km.is the maximum value of substrate concentration corresponding to the half of the value of Vmax

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An enzyme catalyzes a reaction with a Km of 9.50 mM and a Vmax of 2.10 mM · s-1. Calculate the reaction velocity, vo, for each substrate concentration. [S] = 3.25 mM vo : mM · s-1 [S] = 9.50 mM mM · s-1 Vo :
An enzyme is present at 100 nM (nanomolar) and has a Vmax value of 25 uM/s (micromolar/second). The Km for the substrate is 5.2 uM. What is the initial velocity (V0) at a substrate concentration of 15.2 uM? Report your answer to three significant figures in units of uM/s.
An enzyme has a KM = 10 mM and Vmax = 100 mmol/min. Identify the substrate concentration (in mM) in which the velocity will near Vmax when there is a 10-fold decrease in KM.
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Enzyme Kinetics; Author: MIT OpenCourseWare;https://www.youtube.com/watch?v=FXWZr3mscUo;License: Standard Youtube License