Concept explainers
Interpretation:
To which terminal lysine will migrate in an isoelectric focusing experiment with gel of pH gradient
Concept introduction:
Isoelectric point (pI) or isoelectric pH is the pH at which the substance has a charge of zero. An amino acid’s pI is the average of two pKa values involving a zwitterions formation. Isoelectric focusing is a way used to separate amino acids or proteins using pH gradient.
If the pH of the solution is higher than the pI, the substance will deprotonate and will carry negative charge. Conversely, if the pH is lower than pI, the substance will protonate and will carry a positive charge. The substance remains uncharged if pI is equal to the pH and will not migrate to any electrode.
Any ion that has net positive charge (cations) will migrate toward the negatively charged terminal i.e. cathode and the ion with negative charge (anions) will migrate toward positively charged terminal i.e. anode.
Want to see the full answer?
Check out a sample textbook solutionChapter 6 Solutions
Organic Chemistry: Principles and Mechanisms (Second Edition)
- Answer for the number 8 question, Thank you. No need for long explanation.arrow_forwardCircle the main chromophore of the following structure. N OH Narrow_forwardDrag and drop the correct terms in front of their definitions (not all terms are used). 1. the ability of a protein to bind one molecule but not another. specificity 2. the part of a protein molecule that binds the ligand. binding site 3. the ability of a protein to alter its shape to it more closely with that of the ligand. saturation induced fit irreversible inhibitionarrow_forward
- Please answer with mechsnism ASAParrow_forwardpleease answer thisarrow_forwardWhich question will your experiment investigate? (select one and delete the others) How does temperature affect how lactase drops work to break down lactose into glucose and galactose? How does pH affect how lactase drops work to break down lactose into glucose and galactose? How does agitation (movement) affect how lactase drops work to break down lactose into glucose and galactose? What variable will you change in this experiment? This is also called the Independent variable. How will you change it? What variable will you measure in this experiment to determine the effect of the variable you changed? This is also called the Dependent variable. How will you measure it? What variables will you keep the same in this experiment? Check off the items in this list that you would use for this experiment. You may add any items you feel you would need. O skim milk (which contains lactose) O Lactase drops O Graduated cylinder Eye dropper Ice cubes O pH paper O Hot plate Mixing spoon O…arrow_forward
- Which amines can be used to resolve a racemic mixture of amino acids? Check all that apply.Draw the mirror image of the compound shown. Draw the mirror image of the compound shown. Cl Cl |||..arrow_forwardBox the aromatic amino acids, circle the basic amino acids and put a triangle around guanidine. With explanation, please!arrow_forwardLabel all peaks on this NMR of O-ethylsaccharinarrow_forward
- Alanine Distance Traveled Rf value Glycine Phenylalanine Unknown (lower spot) Unknown (upper spot)arrow_forwardAnswer both mcq with reasonarrow_forwardAmong these protein assays, which work in acidic conditions? a. Biuret assay b. Bicinchoninic acid assay c. Bradford assay d. Lowry assayarrow_forward
- Organic Chemistry: A Guided InquiryChemistryISBN:9780618974122Author:Andrei StraumanisPublisher:Cengage LearningChemistry for Today: General, Organic, and Bioche...ChemistryISBN:9781305960060Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. HansenPublisher:Cengage Learning