Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Question
Chapter 33, Problem 12P
Interpretation Introduction
Interpretation:
The sequence of mRNA molecule that can be regulated by given miRNA should be determined.
Concept introduction:
miRNAs (microRNA) are the small RNA molecules, that are non-coding in nature. They can be found in various plants, animals, and viral species. The functions of miRNAs are RNA silencing and gene regulation at the post-transcriptional level.
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Please help with all parts of A, B, C, D
2. You are studying the function of a messenger RNA named Genetixrox and want to label themRNA with a radioactive atom. Assume the mRNA is long and contains all four standardRNA bases. Assume that the cell cannot convert ribonucleotides to deoxyribonucleotides (orvice versa).A. Will you generate radioactive Genetixrox mRNA with 3H-threonine? Threonine is an aminoacid. Answer yes or no, and provide a one sentence rationale.B. Will you generate radioactive Genetixrox mRNA with 3H-adenosine triphosphate? Answeryes or no, and provide a one sentence rationale.C. Will you generate radioactive Genetixrox mRNA with 3H-deoxyadenosine triphosphate?Answer yes or no, and provide a one sentence rationale.D. Will you generate radioactive Genetixrox mRNA 12C-with adenosine triphosphate? Answeryes or no, and provide a one sentence rationale
Polymerase inhibition. Cordycepin inhibits poly(A) synthesis at low
concentrations and RNA synthesis at higher concentrations.
NH2
H.
он
Cordycepin (3'-deoxyadenosine)
a. What is the basis of inhibition by cordycepin?
b. Why is poly(A) synthesis more sensitive than the synthesis of other
RNAS to the presence of cordycepin?
c. Does cordycepin need to be modified to exert its effect?
Be sure to answer all parts.
Write a possible mRNA sequence that codes for each peptide.
a. His-Cys-Tyr-Val-Ser
5¹-
b. Phe-Val-Thr-Tyr-Glu
5'-
5'-
c. Trp-Phe-Asn-Gln
-3'
U
-3'
с
Table 26.2 The Genetic Code-Triplets in Messenger RNA
First Base
(5' end)
-3'
U
UUU
UUC
UUA
UUG
CUU
CUC
CUA
CUG
AUL
Phe
Phe
Leu
Leu
Leu
Leu
Leu
Leu
la
C
UCU
UCC
UCA
UCG
CCU
CCC
CCA
CCG
Second Base
A
UAU
UAC
UAA
UAG
CAU
CAC
CAA
CAG
Ser
Ser
Ser
Ser
Pro
Pro
Pro
Pro
Tyr
55
Tyr
Stop
Stop
His
His
Gin
Gin
G
UGU
UGC
UGA
UGG
CGU
CGC
CGA
CGG
Cys
Cys
Stop
Trp
Arg
Arg
Arg
Arg
Third Base
(3¹ ond)
DUAC DU AG
с
А
А
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- AAAGAGAAAAGAAUA to AAAGAGAAAUGAAUA. Suppose the codon sequence has a single base pair mutation If the old protein sequence was Lys-Glu-Lys-Arg-Ile, what will be the new sequence encoded by the mutant gene? (Use the 3-letter amino acid abbreviations with hyphens and no spaces in between, i.e. Ser-Asn-Tyr-Leu-Pro.) Submit Answer Retry Entire Group No more group attempts remainarrow_forwardTrue or False. Rho-dependent termination of transcription in prokaryotes can take place upon the formation of a strong RNA stern loop in the MRNA just before a run of U residues. True Falsearrow_forwardTrue or False. Explain. A) At no time during protein synthesis does an amino acid make direct contact with the mRNA being translated. B) Because the two strands of DNA are complementary, the mRNA of a gene can be synthesized using either strand as a template.arrow_forward
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