Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 3.3, Problem 12CYP
Explain what happens in positive staining to cause the reaction in the cell.
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The benefits of the negative stain include:
Mark all that apply:
O No shrinkage or distortion of cells due to no heat or
methanol fixing
Because the cells don't take up stain they don't get
shrunk or distorted
O One can more accurately determine cell size and shape
because the cells stand out against the background
One can more accurately determine the cell size and
shape because there is no shrinkage or distortion of cells
O Negatively charged dyes are safer to work with than
positively charged dyes
IMicrosoft B
11:32 A
Explain the relationship between bacterial cell wall with gram-positive and gram-negative in the staining process. Please state chemical used and their function.
This part of the staining process helps the primary stain to remain in the cell during
decolorization.
O 1) Secondary stain
O 2) primary stain
O 3) counterstain
O 4) fixing reagent
O 5) mordant
Chapter 3 Solutions
Foundations in Microbiology
Ch. 3.1 - Explain what unique characteristics of...Ch. 3.1 - Briefly outline the processes and purposes of the...Ch. 3.1 - Name the notable features of microorganisms that...Ch. 3.1 - In one sentence each, define what is involved in...Ch. 3.2 - Prob. 3ELOCh. 3.2 - Prob. 4ELOCh. 3.2 - Prob. 5ELOCh. 3.2 - Prob. 6ELOCh. 3.2 - Prob. 7ELOCh. 3.2 - Prob. 8ELO
Ch. 3.2 - Prob. 3CYPCh. 3.2 - Prob. 4CYPCh. 3.2 - Prob. 5CYPCh. 3.2 - Prob. 6CYPCh. 3.2 - Prob. 7CYPCh. 3.2 - Prob. 8CYPCh. 3.2 - Prob. 9CYPCh. 3.2 - Compare the way that the image is formed in the...Ch. 3.3 - Prob. 9ELOCh. 3.3 - Define dyes and describe the basic chemistry...Ch. 3.3 - Prob. 11ELOCh. 3.3 - Distinguish between simple, differential, and...Ch. 3.3 - Describe the process of Gram staining and how its...Ch. 3.3 - Prob. 11CYPCh. 3.3 - Explain what happens in positive staining to cause...Ch. 3.3 - Prob. 13CYPCh. 3.3 - For a stain to be considered differential, what...Ch. 3.3 - Prob. 15CYPCh. 3.4 - Prob. 14ELOCh. 3.4 - Prob. 15ELOCh. 3.4 - Explain what an isolated colony is and indicate...Ch. 3.4 - Differentiate between a pure culture, subculture,...Ch. 3.4 - What kinds of data are collected during...Ch. 3.4 - Prob. 19ELOCh. 3.4 - Prob. 16CYPCh. 3.4 - Prob. 17CYPCh. 3.4 - Prob. 18CYPCh. 3.4 - Compare and contrast three common laboratory...Ch. 3.4 - Describe how an isolated colony forms.Ch. 3.4 - Prob. 21CYPCh. 3.5 - Prob. 20ELOCh. 3.5 - Name the three general categories of media, based...Ch. 3.5 - Compare and contrast liquid, solid, and semisolid...Ch. 3.5 - Prob. 23ELOCh. 3.5 - Prob. 24ELOCh. 3.5 - Identify the qualities of enriched, selective, and...Ch. 3.5 - Explain what it means to say that microorganisms...Ch. 3.5 - Describe live media and the circumstances that...Ch. 3.5 - Describe the main purposes of media, and compare...Ch. 3.5 - Differentiate among the ingredients and functions...Ch. 3.5 - Explain the two principal functions of dyes in...Ch. 3.5 - Why are some bacteria difficult to grow in the...Ch. 3.5 - What conditions are necessary to cultivate viruses...Ch. 3.L1 - Prob. 1MCQCh. 3.L1 - Prob. 2MCQCh. 3.L1 - Prob. 3MCQCh. 3.L1 - Prob. 4MCQCh. 3.L1 - Prob. 5MCQCh. 3.L1 - Prob. 6MCQCh. 3.L1 - Resolution is _____________ with a longer...Ch. 3.L1 - Prob. 8MCQCh. 3.L1 - Prob. 9MCQCh. 3.L1 - The specimen for an electron microscope is always...Ch. 3.L1 - Motility is best observed with a a. hanging drop...Ch. 3.L1 - Prob. 12MCQCh. 3.L1 - Prob. 13MCQCh. 3.L1 - Prob. 14MCQCh. 3.L1 - What type of medium is used to maintain and...Ch. 3.L1 - Prob. 16MCQCh. 3.L1 - Multiple Matching. For each type of medium, select...Ch. 3.L1 - Prob. 1CSRCh. 3.L1 - Prob. 2CSRCh. 3.L1 - Prob. 3CSRCh. 3.L1 - Prob. 1WCCh. 3.L1 - Prob. 2WCCh. 3.L1 - Prob. 3WCCh. 3.L1 - Describe the steps of the Gram stain, and explain...Ch. 3.L1 - Describe the steps you would take to isolate,...Ch. 3.L1 - Prob. 6WCCh. 3.L1 - Evaluate the following preparations in terms of...Ch. 3.L2 - A certain medium has the following composition: a....Ch. 3.L2 - a. Name four categories that blood agar fits. b....Ch. 3.L2 - Prob. 3CTCh. 3.L2 - Go back to section 1.2 and observe the six...Ch. 3.L2 - Since the dyes for the Gram stain are not specific...Ch. 3.L2 - Examine figure 3.10a, b (shown here). If you...Ch. 3.L2 - Prob. 2VC
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- The benefits of the negative stain include: Mark all that apply: 1. No shrinkage or distortion of cells due to no heat fixing 2. Because the cells don't take up stain they don't get shrunk or distorted 3. One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells 4. Negatively charged dyes are safer to work with than positively charged dyes 5. One can more accurately determine cell size and shape because the cells stand out against the backgroundarrow_forwardExplain the difference between Gram positive and Gram negative cell walls. Describe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?) How is the Gram stain used clinically to help identify the cause of an infection?arrow_forwardWhat is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? Paragraph BIUarrow_forward
- Which step is the most crucial or most likely to cause poor results in the Gram stain?arrow_forwardA student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 10 mm) in diameter, and wants to viewa magnified image of a cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? 10X O 40X O 100X 0 4Xarrow_forwardStaining of cells is often performed to enhance images acquired by brightfield microscopy. Phasecontrast microscopy does not require cell staining. Why is this advantageous?arrow_forward
- What stain would you use to differentiate between bean and ant cells? Explain and include your expected results.arrow_forwardDescribe what happens at each step in the Gram stain.arrow_forwardStaining is often needed to distinguish the structure of cells as most cells are almost transparent. * true * falsearrow_forward
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