The effect of an increased Cro concentration on the expression of the λ repressor gene should be determined. The effect of an increased λ repressor gene on the Cro gene expression should be determined. The effect of an increased λ repressor on the expression of lambda repressor gene should be determined. Concept introduction: The lytic cycle is the rupture of the host cell, resulting in the release of a number of viruses. Bacterial viruses undergo lytic cycle only and are termed as the virulent bacteriophages. These viruses are the cause of their host cells death. Another life cycle of bacteriophage includes a lysogenic cycle. In this cycle, the viral DNA remains in the inactivated state even after various reproduction cycles of the host bacterium. After a certain time only, the viral DNA is released from the host’s cell DNA and undergoes lytic cycle.

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Cell signaling The maximum secretion rates of proteins are one type of limiting constraint on signal-ing processes since a cell cannot send a signal faster than it can secrete it. These maximum signalingrates can be estimated with maximum rates of transcription and translation. Consequently, proteins withstrong promoters can be synthesized at much higher rates. Immunoglobulins, which are important signal-ing molecules, have very strong promoters. Their maximum secretion rate is on the order of 2000 to 8000antibody molecules/cell/second, which corresponds to approximately 1 pg/cell/hr. Part AWhat would be the maximum signal (in pg/cell/hr) that a cell could send for the following situations: (1)Signal molecule A has a MW = 100,000 daltons and is secreted at a rate of 1000 molecules/cell/second. (2)Signal molecule B has a MW = 50,000 daltons and is secreted at 5000 molecules/cell/second.Part BWhich molecule is secreted at a higher rate? By how much?Part CIf equivalent fluxes of both…

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Chapter 32, Problem 9P

Interpretation Introduction

Interpretation:

The effect of an increased Cro concentration on the expression of the λ repressor gene should be determined.

The effect of an increased λ repressor gene on the Cro gene expression should be determined.

The effect of an increased λ repressor on the expression of lambda repressor gene should be determined.

Concept introduction:

The lytic cycle is the rupture of the host cell, resulting in the release of a number of viruses. Bacterial viruses undergo lytic cycle only and are termed as the virulent bacteriophages. These viruses are the cause of their host cells death. Another life cycle of bacteriophage includes a lysogenic cycle. In this cycle, the viral DNA remains in the inactivated state even after various reproduction cycles of the host bacterium. After a certain time only, the viral DNA is released from the host’s cell DNA and undergoes lytic cycle.

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Cell signaling The maximum secretion rates of proteins are one type of limiting constraint on signal-ing processes since a cell cannot send a signal faster than it can secrete it. These maximum signalingrates can be estimated with maximum rates of transcription and translation. Consequently, proteins withstrong promoters can be synthesized at much higher rates. Immunoglobulins, which are important signal-ing molecules, have very strong promoters. Their maximum secretion rate is on the order of 2000 to 8000antibody molecules/cell/second, which corresponds to approximately 1 pg/cell/hr. Part AWhat would be the maximum signal (in pg/cell/hr) that a cell could send for the following situations: (1)Signal molecule A has a MW = 100,000 daltons and is secreted at a rate of 1000 molecules/cell/second. (2)Signal molecule B has a MW = 50,000 daltons and is secreted at 5000 molecules/cell/second.Part BWhich molecule is secreted at a higher rate? By how much?Part CIf equivalent fluxes of both…

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