The dissociation constant of lambda repressor-DNA complex and standard free energy of binding should be determined. Concept introduction: Theλ repressor in activated state binds to O R 1 and O R 2 sites and inhibits the transcription of phage gene. On the other hand, Cro protein binds to the same sites, to which λ repressor binds. In this way, the Cro protein inhibits the action of λ repressor.

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Chapter 32, Problem 14P

Interpretation Introduction

Interpretation:

The dissociation constant of lambda repressor-DNA complex and standard free energy of binding should be determined.

Concept introduction:

Theλ repressor in activated state binds to O_R1 and O_R2 sites and inhibits the transcription of phage gene. On the other hand, Cro protein binds to the same sites, to which λ repressor binds. In this way, the Cro protein inhibits the action of λ repressor.

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