Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 30, Problem 30.9QAP
Interpretation Introduction
Interpretation:
The order of elution of protein ions A2+, B+ and C3+ needs to be determined, if they are ionized at pH at which electrical FFF separation is carried out.
Concept introduction:
FFF or Field flow fractionation is a separation method in which a field is applied to solution that is pumped through channel perpendicular to the direction of flow such that the particles separate. The separation depends on the force applied by the field.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Three large proteins are ionized at the pH at which an electrical FFF separation is carried out.
(i) if the ions are designated A2+, B2+, and C3+, predict the order of elution.
(ii) What is FFF?
(iii) List the four subtechniques of FFF.
An amino acid on the surface of a protein was labelled covalently with 1.5-I AEDANS and another was labelled covalently with FITC. The fluorescence quantum yield of 1.5-I AEDANS decreased by 10 per cent due to quenching by FITC. What is the distance between the amino acids? (Refer to Table 17G.3 for the appropriate value of R0.)
The volume of O2(g) adsorbed on a surface of 1 g of a silica sample at 5°C is V1=1.4 cm3 at P1=95.1 torr and V2=10.6 cm3 at P2=760 torr. Identify the maximum volume of oxygen, Vm, that can be adsorbed. Consider the Langmuir isotherm model
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Similar questions
- Determine the final concentration in mol dm–3 of K+ and SO42– when the following three solutions in volumetric flasks are mixed together: 500 cm3 of 0.20 mol dm–3 KAl(SO4) 2; 1000 cm3 of 0.050 mol dm–3 K2SO4 and 500 cm3 of water. The concentration of K+ is Select one: a. 0.20 × 103 mol dm–3 b. 0.20 mol dm–3 c. 0.10 × 10–3 mol dm–3 d. 0.10 mol dm–3arrow_forwardThere is a class of polyesters that have the general structure shown below. For example, in polyethylene terephthalate (PET) m =1. How would you expect the Tg ad Tm of these polymers to vary with m, given that the molecular weight of the chain is held constant? Give reasons for your answer. Also, assuming that you are now just considering one member of this class of polymers, say PET, how does the Tg vary with molecular weight, n.arrow_forwardCalculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.arrow_forward
- Suppose the concentration of a solute decays linearly along the length of a container according to c(x) = c0 - αc0x, where c0 is the concentration at x = 0. Calculate the thermodynamic force on the solute at 25 °C and at x = 10 cm and 20 cm given that the concentration falls to 1/2c0 when x = 10 cm. Hint: Start by finding the value of α.arrow_forwardA student conducted an experiment to determine the concentration of benzene in an ethanol solution. Use the UV spectrum of the solution in a 2.0 cm cell. Within the spectrum, there was a peak attributed to benzene at 260 nm, with an absorption of 0.69. In a reference source, the student found that the adsorption of the benzene strip with ethanol was 2.95 x 10-3 ppm cm-. What is the molar concentration of benzene in the sample (mm of benzene = 78.11 g/mol)arrow_forwardDetermine the final concentration in mol dm–3 of K+ and SO42– when the following three solutions in volumetric flasks are mixed together: 500 cm3 of 0.20 mol dm–3 KAl(SO4) 2; 1000 cm3 of 0.050 mol dm–3 K2SO4 and 500 cm3 of water. The concentration of K+ isarrow_forward
- What is involved in Phases l and Phases ll of biotransformation?arrow_forwardThe activity of an enzyme requires a glutamic acid to display its –COOH functional group in the protonated state. Suppose the pKa of the -COOH group is 4.07. What fraction of the enzymes will be active at pH = 4.07? 25% O 75% O 50% 100% 0% Submit Previous Answers Completed Part C At what pH will the enzyme show 80% of maximal activity? Express your answer using two decimal places. ν ΑΣφ pH = Submit Request Answerarrow_forwardThe biuret reaction is a general method for the determination of proteins or peptides. This method is based on the reaction of the Cu2+ with four NH groups of the peptide bonds, forming a violet complex with an absorption maximum at 540 nm. The intensity of the color is proportional to the number of peptide bonds present, and therefore to the amount of protein. This reaction is specific, the dipeptides and amino acids give a negative reaction, and few substances interfere. A quantity of 19.89 g of fish meat is extracted with 100 mL of glacial acetic acid. Subsequently, 3 mL of the previous solution with 3 mL of the biuret reagent to generate the reaction, after 30 minutes. To reduce errors due to other interfering substances and residual turbidity, the corrected absorbance method Ac is applied to 500 nm and 570 nm. In a study, a standard sample analyzed by the Kjeldahl method (total Nitrogen analysis) is obtained, resulting in 0.43% N (w / v), (Protein content [%] =% N x 6.38). 3 mL of…arrow_forward
- The following data are for the chemisorption of hydrogen on copper powder at 25°C. Confirm that they fit the Langmuir isotherm at low coverages. Then find the value of K for the adsorption equilibrium and the adsorption volume corresponding to complete coverage. \ table V 3 [la, 25, 129,253, 540],[m², 0.042, 0.163,0.221,0.32|]] сarrow_forwardAssume a pH gradient of 4.0 units across a thylakoid membrane, with the lumen more acidic than the stroma.The intensity of natural sunlight drops off dramatically beyond 650 nm.Calculate the maximum number of moles of protons that could be pumped against the gradient by the energy in a mole of photons of 650 nm wavelength. Assume 100% efficiency and T = 25 °C for this calculation.arrow_forward1. Calculate the Coulombic (ionic) interaction energy between atoms for the C=O ∙∙∙H-N hydrogen bond in a peptide at a distance of 1.5Å in units of kJ/mol. Assume that the partial charge on O is −0.434, the partial charge on H is +0.417 and the permittivity of the protein environment is 6. coulimbic interaction energy= 27.95 kJ/mol 2. The dipole moment of an individual peptide group is approximately 3.46 D. Assuming that the dipoles line up linearly estimate the energy of interaction (in kJ.mol) of the hydrogen bond in Q1 using a dipole-dipole model. *******please answer #2**********arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning
Principles of Instrumental Analysis
Chemistry
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Cengage Learning