Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
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Chapter 30, Problem 30.3QAP
Interpretation Introduction
Interpretation:
The effect of pH on separation of amino acids during electrophoresis process needs to be explained.
Concept introduction:
Electrophoresis is a technique of separation of ions by applying electric field. In this method, the analytes can be separated through conductive medium in presence of electric field. Without this effect the cations migrate towards the negatively charged cathode and anions moves toward positively charged anode.
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. Describe the pH range of acceptable buffering behavior for the amino acids alanine, histidine, aspartic acid, and lysine.
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- Chemistry a. Are there methods other than spectrophotometry to determine protein concentration? If there is, please provide an explanation of this method along with its advantages and disadvantages! b. Explain the principle of protein separation by electrophoresis method! c. Can protein electrophoresis method be used to characterize proteins? Please explain why!arrow_forwardMost of the ultraviolet absorption of proteins at 280 nm is due to their content of a. Glutamate b. Tryptophan c. Alanine d. Aspartatearrow_forwardThe first step in the procedure asks you to prepare five dilutions of the protein standard (BSA) in the range of 0.2 to 1.0 mg/ml. For each concentration, a volume of 1.0 mL should be prepared. Describe how you will prepare these standards using a stock solution of BSA that is 2.0 mg/ml.arrow_forward
- Differentiate and explain the chemical basis of the Kjeldahl, Lowry and Bradford techniques that can be used to quantitate proteins in quality control and research.arrow_forwardFor the amino acid alanine, the major species in solution at pH 7 is the zwitterionic form, which has a negatively charged carboxylic acid group and a positively charged amino group. There is a less common neutral form in which neither group is charged. The carboxylic acid group of alanine has a p?a of 3 The amino group of alanine has a p?a of 8 Estimate the ratio of the concentration of the neutral amino acid species to the zwitterionic species at pH 7. __?__x10^?arrow_forward22-49 Based on your knowledge of the chemical properties of amino acid side chains, suggest a substitution for leucine in the primary structure of a protein that would probably not change the character of the protein very much.arrow_forward
- 22-85 Denaturation is usually associated with transitions from helical structures to random coils. If an imaginary process were to transform the keratin in your hair from an (-helix to a (-pleated sheet structure, would you call the process denaturation? Explain.arrow_forward22-62 Distinguish between intermolecular and intramolecular hydrogen bonding between backbone groups. Where in protein structures do you find one, and where do you find the other?arrow_forward22-20 Show how alanine, in solution at its isoelectric point, acts as a buffer (write equations to show why the pH does not change much if we add an acid or a base).arrow_forward
- 22-61 Polyglutamic acid (a polypeptide chain made only of glutamic acid residues) has an a-helix conformation below pH 6.0 and a random-coil conformation above pH 6.0. What is the reason for this conformational change?arrow_forward22-53 Do iron and zinc ions play role in protein structure? If so, what is the role for either or both?arrow_forward22-9 What is the difference in structure between tyrosine and phenylalanine?arrow_forward
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