Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
bartleby

Concept explainers

bartleby

Videos

Question
Book Icon
Chapter 30, Problem 23P
Interpretation Introduction

(a)

Interpretation:

Nucleolus of 3 different tissue that is brain, liver and muscle are collected and allowed to undergo transcription. The RNA was applied to DNA chips. The cause of intensity of hybridization differing from gene to gene is to be described.

Concept introduction:

DNA chip technology is also known as the DNA microarray technology. In this process different genes or piece of DNA are collected in a microarray. These genes are attached to a solid surface. When the sample of polynucleotide strand is added upon it, due to complementary nature of nucleotides specific polynucleotide strand it will attach to its complementary strands only. The intensity of shading helps in identifying which polynucleotide stands were there in the sample.

Interpretation Introduction

(b)

Interpretation:

The cause of different hybridization pattern of same RNA in different tissue is to be described.

Concept introduction:

DNA chip technology is also known as the DNA microarray technology. In this process different genes or piece of DNA are collected in a microarray. These genes are attached to a solid surface. When the sample of polynucleotide strand is added upon it, due to complementary nature of nucleotides specific polynucleotide strand will attach to its complementary strands only. And the intensity of shading helps in identifying which polynucleotide stands were there in the sample.

Interpretation Introduction

(c)

Interpretation:

The cause of expression of some genes in all the tissues are to be described.

Concept introduction:

DNA chip technology is also known as the DNA microarray technology. In this process different genes or piece of DNA are collected in a microarray. These genes are attached to a solid surface. When the sample of polynucleotide strand is added upon it due to complementary nature of nucleotides specific polynucleotide strand will attach to its complementary strands only. And the intensity of shading helps in identifying which polynucleotide stands were there in the sample.

Interpretation Introduction

(d)

Interpretation:

Cause of addition of inhibition inhibitor in sample is to be proposed.

Concept introduction:

DNA chip technology is also known as the DNA microarray technology. In this process different genes or piece of DNA are collected in a microarray. These genes are attached to a solid surface. When the sample of polynucleotide strand is added upon it due to complementary nature of nucleotides specific polynucleotide strand will attach to its complementary strands only. And the intensity of shading helps in identifying which polynucleotide stands were there in the sample.

Blurred answer
Students have asked these similar questions
Question. What would the forward primer sequence look like if it were intended to bind the area of the DNA template?
Original sequence:    Consider the following coding 71 nucleotide DNA template sequence (It does not contain a translational start):  5’-GTTTCCCCTATGCTTCATCACGAGGGCACTGACATGTGTAAACGAAATTCCAACCTGAGCGGCGT GTTGAG-3’    Question:    4) In a mutant you discovered that the underlined nucleotide has  been deleted. What would the resulting peptide sequence be? What  type of mutation is this?  5’-GTTTCCCCTATGCTTCATCACGAGGGCACTGACATGTGTAAACGAAATTCCAACCTGAGCGGCGT GTTGAG-3
Instruction - Please answer them correctly - Please answer all of them, they are connected. MUTATION Fill in the correct nucleotide base pairing and amino acid sequence of the mutated DNA a. What is the 3’-5’ DNA sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) b. What is the mRNA sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) c. What is the tRNA sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) d. What is the amino acid sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) e. What is the most convincing type of mutation had occurred? (Frameshift resulting Missense; Frameshift resulting Nonsense; Substitution – Silent; Substitution – Missense; Substitution – Nonsense)
Knowledge Booster
Background pattern image
Biochemistry
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
  • Text book image
    Biochemistry
    Biochemistry
    ISBN:9781305577206
    Author:Reginald H. Garrett, Charles M. Grisham
    Publisher:Cengage Learning
Text book image
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
DNA vs RNA (Updated); Author: Amoeba Sisters;https://www.youtube.com/watch?v=JQByjprj_mA;License: Standard youtube license