Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Related questions
Concept explainers
Question
Transcribed Image Text:Calculating Transformation Efficiency
Transformation Efficiency is defined as the number of colony forming units (cfu) which
would be produced by transforming 1 ug of plasmid DNA into a given volume of competent
cells.
Transformation Efficiency (TE) = number of cfu/ug DNA
You have just transformed 1 ul (100 pg/ul) of control pWasabi (plasmid) DNA into 50 µl of
E. coli DH5a by the heat-shock method. You then add 950 µl of SOC medium to outgrow the
bacteria. Of this, you plated 50 µl onto a LB plate containing ampicillin. After overnight
incubation, you observed that there are 150 colony forming units (cfu) on the plate. Calculate
the transformation efficiency (show your step-wise calculations below).
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution
Trending nowThis is a popular solution!
Step by stepSolved in 2 steps with 1 images
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Transformation of pGLO in E. coli: 8) Explain your expected results on LB, LB+Amp, and LB+Amp+Arabinose plates for the -pGLO and the +pGLO treatments. (6 total results, please assume everything works) Please describe how much growth, and whether if will or won’t glow.arrow_forwardBailey and Ollis Biochemical Engineering (Write your solution)arrow_forwardBiomedical Engineering- Bridging Medicine and Technology -- W. Mark Saltzman 2009 In Section 9.2, the text discusses the zero-order kinetics of alcohol disappearance from the blood. a. Using the approach outlined in section 7.2.2 (Tracer balance in the body), find an equation describing the concentration of alcohol in the plasma versus time, given that the initial concentration in the plasma (at time=0) is 10 mM.(Refer to Figure 9.5, the half-life of alcohol in plasma is 150 min) b. Graph the results of (a). c. Over what time interval is the expression that you derived in (a) valid? Why is it not valid for all time?arrow_forward
- GEL FILTRATION: What would you expect to be the effects on (a) the height of the peaks and (b) the volume between the peaks if the length of the Sephadex column were doubled? Please explain.arrow_forwardDo not give handwriting solution.arrow_forwardWhat biochemical function do these Omega-3 fats play? Much cheaper alternative sources of omega-3 fats include fats from some seeds and nuts (most often from flax seed in consumer products), but the allure of fish oil sources maintains these products' popularity despite much higher prices. Is there a difference in the omega-3 fats derived from plant and animal sources? If so, explain the difference and why one is "better" than the other.arrow_forward
arrow_back_ios
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education, 
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education