Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 3, Problem 7MCQ
Assume you stain Clostridium by applying a basic stain, carbolfuchsin, with heat, decolorizing with acid-alcohol, and counterstaining with an acidic stain, nigrosin. Through the microscope, the endospores are _____1______, and the cells are stained ___2____ .
- 1. 1—red: 2—black
- 2. 1—black: 2—colorless
- 3. 1—colorless: 2—black
- 4. 1—red: 2—colorless
- 5. 1—black: 2—red
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Which parts of the modern microscope do you think were absent from Leeuwenhoek’s microscope?
Why is it necessary to calibrate the ocular micrometer with each objective?
When measuring dimensions of microorganisms, what slide preparation should be used and why?
Complete the following for each objective in your microscope:
_____ Ocular micrometer divisions = _________ stage micrometer divisions
_____ Ocular micrometer divisions = _________1 stage micrometer division = _____mm
Microscopic Measurements 1
Arrange the following items by using numbers (smallest) to 8 (largest).
______ AIDS virus ______ rickettsia
______ worm ______ white blood cell (WBC)
______ amoeba ______ protein
______ staphylococci…
An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present?
No acid-fast bacteria are present on the slide
Methylene blue was not used
Carbolfuchsin was not used
Acid-alcohol was not used
Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)
a
acid alcohol
b
crystal violet
c
carbolfuschin
d
malachite green
e
safranin
f
iodine
g
alcohol
h
methylene blue
Chapter 3 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Complete the following table with your observations from the prepared slide Name of the Bacteria Type of Endospore Staining Technique (+ or −) Drawing Clostridium botulinum Clostridium perfringens Bacillus anthracisarrow_forwardAn acid-staining method was performed on a bacterial smear using the dyes carboifuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following couid explain why only blue cells were present?arrow_forwardThe image is showing Bacillus subtillis bacteria under 400x magnification, the same magnification used on the plant and animal photos. Why are the bacterial cells so much harder to see in this microscope image?arrow_forward
- 1-What can occur if you quickly drop the coverslip onto your wet mount? 2-How can one add stain to the wet mount that has already been prepared? 3-Some live microorganisms can move very quickly. How do we slow these down to view in the microscope?arrow_forwardComplete for Additional Differential Staining B: Negative staining ~ thin smear C: Endospore Stainarrow_forwardThe following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope at 10x magnification. Samples were stained with Lugols solution for better viewing. Differentiate these two imagearrow_forward
- In a Gram-stained smear, medium-sized rods with rounded, pink ends were found, arranged chaotically. 1. What are the tinctorial properties of the bacteria from which the smear is made? 2. What is the purpose of studying the tinctorial properties of microorganisms? 3. How to stain the slide by the Gram method?arrow_forward1. Why is it important to use a small amount of bacteria when making a smear? Give at least 2 reasons. 2. What are the two reasons for “fixing” a smear? 3. Simple staining is useful for determining what three characteristics of a bacterial cell? 4. With respect to bacteria, define morphological arrangement. Explain thoroughly!arrow_forwardPicture in white is acid fast other picure is negative stain so far this is the information I have: gram stain: positive motility: motile oxygen requirement: Facultative anaerobe and than I need these two acid fast stain: _____??? negative stain: _____????arrow_forward
- An acid-fast stain can be used to detect protective coverings found in using light microscopy. organisms incorporating mycolic acid in the cell wall organisms forming endospores organisms with capsules organisms with Gram-negative cell wallsarrow_forwardEndospores can be seen as refractile structures in unstained cells and as colorless areas in Gram-stained cells. Why is it necessary to do an endospore stain to verify the presence of endospores?arrow_forwardDraw what you would expect to see for acid fast stain and endospore stain procedure if you did not stain with counterstain. Color pencils need to be used.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Bacterial Structure and Functions; Author: Osmosis;https://www.youtube.com/watch?v=b15Hy3jCPDs;License: Standard youtube license