Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 3, Problem 4MCQ
Summary Introduction
Introduction: Chlorophyll is a green pigment present in chloroplasts of plants, algae, and cyanobacteria. This pigment absorbs light during photosynthesis and converts it to photochemical energy.
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THIS IS A MULTIPART QUESTION, PLEASE ANSWER ALL QUESTION
Using the image:
5. Identify whether the organism is a green alga, a diatom, a dinoflagellate, Eubacterium, or a Cyanobacterium. Explain the characteristic(s) that led you to that identification.
6. a. What type of microscopy (brightfield, darkfield, or phase contrast) was to obtain the image? Explain how you can tell.
b. Name a different type of microscopy would be also appropriate to use to visualize the organism in the image. How would the image change if you used that type of microscopy instead?
7. The image was taken using a microscope that has 10X, 40X, and 100X objectives. What is a possible magnification for the ocular lens on this microscope? Explain your response or show calculations.
The series of photos above shows you plant cells when looked at with a light microscope. The first image is taken with a 4x objective lens. We always look through an ocular lens (eyepiece of a microscope) which in this case has a 10x magnification. This means tha the total magnification for the first image is 40x (meaning 40 times as big as you could see with the naked eye). The second image is using the 10x lens, with a total magnification of 100x. The third image is taken using a 40x objective, with a total magnification of 400x!
What does each box in the microscope image represent?
A. each box is an organelle
B. each box is an animal cell
C. each box is a plant cell
D. each box is a nucleus
Image A:
Image B:
25 um
O A. image A- scanning electron microscope, Image B- phase contrast microscope
O B. Image A brightfield microscope; Image B- darkfield microscope
O C.image A- brightfield scanning microscope Image B- phase contrast scanning microscope
D. image A- transmission electron microscope, Image B- scanning electron microscope
O E. image A- scanning electron microscrope, Image B- transmission electron microscope
Chapter 3 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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- A student missed the laboratory period where the use of the microscope was demonstrated. The instructor asked the student to read the description in the laboratory manual and then proceed to examine bacterial cells with the oil immersion lens. The student skimmed the directions and began. After about 15 minutes of struggling, the student gave up in despair without seeing anything. Below is a detailed description of what the student did. How many mistakes did the student make and why didn’t the student see anything? a. Plugged in the microscope and turned the light source to maximum intensity. Made a wet mount and placed it on the stage with the low-power objective lens in position. Tried to focus with the coarse adjustment, but decided the bacteria were too small and needed to be seen with the high-power objective lens. Rotated the high-power objective lens into position, but saw the lens would likely touch the slide, so lowered the stage so that the objective lens rotated freely.…arrow_forwardPlease refer to the image below. Which of the following will leave no stain nor grease on paper after a few minutes of exposure to air? Click all answers that apply Which of the following will leave a stain but NOT grease on paper after a few minutes of exposure to air? Click all answers that apply Which of the following will turn the paper translucent? Click all answers that applyarrow_forward4. If the diameter of the field of view (FOV) for your microscope is 5.5 mm at 40x total magnification, what is the diameter of the FOV at 1000X? Show work and don't forget units! 5. Use the counting method to estimate the length of an elodea cell. You will need to use your calculation for the FOV at 400x total magnification. Show work and don't forget units! Elodea Leaf at 400x total mag 6. Use the percent method to estimate the size of a human cheek epithelial cell. You will need to use your calculation for the FOV at 1000x total magnification. Show work and don't forget units! Cell Membrane Nucle usarrow_forward
- The image in Figure 2 is of the diatom, Fragilariopsis cylindrus. Determine the size (length) of the diatom indicated by the red arrow and determine the magnification Show your calculations.arrow_forwardThe benefits of the negative stain include: Mark all that apply: 1. No shrinkage or distortion of cells due to no heat fixing 2. Because the cells don't take up stain they don't get shrunk or distorted 3. One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells 4. Negatively charged dyes are safer to work with than positively charged dyes 5. One can more accurately determine cell size and shape because the cells stand out against the backgroundarrow_forwardMake a biological diagram using this picture which has 400 X Magnification. I also added an example of this, you could trace and draw. Follow these guidelines: Guidelines for proper drawings1. Use blank unlined paper and a sharp pencil. No pens and no colour.2. A TITLE is at the top of the page, is descriptive centered underlined printed ALL CAPS.3. Drawings/photographs should be large enough to show all parts without crowding. The greater the number of parts to be included, the larger the drawing/photograph should be. In general, drawings/photograph should be ½ to ¾ of a page in size.4. Keep the drawing to the left of the centre of the page.5. Observe your specimen carefully, noting all the details of the specimen.6. Draw only what you see, not what you think should be there.7. Drawings should be as simple as possible, with clean-cut lines showing what has been observed. Do not “sketch”.8. Do not shade. Use a stippling technique for darker areas.9. All labels should be in a column to…arrow_forward
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