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Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
Endospore staining:
Endospore staining is a method used to selectively stain bacterial endospores. This method was first published in 1922 by Dorner and was later modified by Shaeffer and Fulton. This method helps in distinguishing between the spore-forming bacteria from the non-spore-forming ones.
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- Define the following (Microbiology) Lag phase Exponential phase Stationary phase Death phase= Q Set up Bacteria Growth Experiment 2024 Saved Search Design Layout References Mailings Review View Help Table Design Layout (Body) 11 V A A Aa PEET I Uab x, x AA Font √√ Data Collection Table 1-3: just bacteria sample Day General Observations (color, texture, coverage) 2 No obvious growth. 6 Paragraph V עו # of % Photo of plate colonies Coverage 0% Colors range from white, to light grey, size being roughly ½ cm circles. 1-3 3.65% 2-3 3-3 Large amount of growth since day 4 circles ranging from white grey, black, green, and pink have been observed. White and black areas are about 2 1/2cm around while pink, and green are 1/2cm around. There is also many small white dots covering the majority of the surface area. LX Text Predictions: On Accessibility: Investigate 75° COLLEGE 1-4 40% 2-4 3-3 W FB B Normal No Spacing Heading Heading 2 Title Subtitle Subtle Emphas F7 FB SAMSUNG >/11 D Styles 8 Circles ranging from white grey, black, 1-5 green, and pink have been observed getting bigger.…SMART ll A Or © X39% 011:23 Q1WK2.docx 团: Biotechnology Quarter 1 Week 2 Name (Surname, First Name, Middle Initial): Section (Program – Grade Level – Section): Mini Home Experiment Materials: o perfume o clean drinking glass o water o tomato/kamatis o ginger o salt O sugar o platito o knife Procedure: Set-up A: 1. Open your bottle of perfume and spray it at one corner of your bedroom. 2. Observe what happens after 15-20 minutes. Questions: 1. What happened after 15-20 minutes? 2. What is the reason behind this phenomenon? Set-up B: (Be careful with the use of sharp objects. ADULT SUPERVISION IS ADVISED) 1. Cut two ginger sticks of the same size. 2. Prepare two drinking glass of the same size and fill it with equal amounts of water. Label them A and B. 3. Dissolve three teaspoon of salt in glass B. 4. Place the ginger sticks separately in glass A and glass B. 5. Leave the set-ups overnight. 6. Observe the ginger sticks the next day. Questions: 1. Which is the control set-up? 2. What did…
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- In the this figure showing a microbial death curve, what is the Decimal Reduction Time for the microbe being killed? Plotting the typical microbial death curve logarithmically (red line) results in a straight line. Log10 of number of surviving cells 6.0 5.0 4.0 3.0 2.0 1.0 1 One log decrease = 90% of population killed 3 Time (min) 2 3 minutes 6 minutes 1 minute 24 minutes 4 5 T 6 1,000,000 750,000 500,000 250,000 100,000 Arithmetic number of surviving cells Cannot be determined from graphA researcher analyzes unpasteurized apple juice suspecting E. coli contamination of the juice. He/she plates out 0.1 mL of a 10-2 dilution of juice on each plate and grows up the plates. He/she then counts colonies on each plate. Plate 1 – 242 colonies Plate 2 – 237 colonies Plate 3 – 249 colonies Calculate the CFU/mL for this juice. Show formula used and include unitsMy professor instructed me to make presentation on this topic "Periodontal microbes interaction with FISH". I have to make 4 power point slides regarding this topic. Can you give some information about the topic which i can use in the slides? Also provide some necessary relevant diagram or image regarding the topic. Please answer at your own easy words. If you do so i will rate you positive, Thank you.
- A researcher analyzes unpasteurized apple juice suspecting E. coli contamination of the juice. He/she plates out 0.1 mL of a 10-2 dilution of juice on each plate and grows up the plates. He/she then counts colonies on each plate. Plate 1 – 242 colonies Plate 2 – 237 colonies Plate 3 – 249 colonies a. Calculate the CFU/mL for this juice. Show formula used and include units. b. If acceptable levels of E. coli in juice were 0.02 CFU/ml juice, would this juice be acceptable for human consumption? Why or why not?Question 1 Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You’re concerned that it’s an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium or a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. What is one experiment you could do, involving culturing the organism? Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we have done/could do in our lab. What is a procedure you could do, involving making a slide of the organism? Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we have done/could do in our lab. Without testing anything, how do you know this is not a viral infection?Researchers discover a new strain of a previously known bacterial organism and need help understanding properties related to it's bacterial growth patterns. The new strain is lethal to humans and is found to be increasingly deadly in arctic climates. Researchers have also provided the following chart of plate counts: Colony |(minutes) Forming Units by ml (CFU/ml) Time 20 15 20 30 340 45 50,009 60 175,168,185 90 175,168,189 120 175,168,002 150 423,237 210 505 | 270 5 a) Create a bacterial growth curve with log values of CFU/ml on the Y-axis andthe time on the x axis. b) Indicate the different phases of the growth curve on your plot. c) Determine the growth constant value (k) and the Generation Time value (G). show calculations 8 8