Microbiology: An Introduction (13th Edition)
Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 3, Problem 2A
Summary Introduction

To write:

The use of a good compound light microscope with a resolving power of 0.3 mm, a 10x and a 100x oil immersion lens.

Given:

“Compound light microscope” and “resolving power”

Introduction:

A compound microscope is consists of two convex lenses with shorter focal length, used to magnify the tiny objects. The resolving power of the compound microscope is 0.2µm and magnification power is 2000x.

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Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X oil immersion lens. Would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm?
Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.
Calculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4.5 mm = 4,500 um Scanning (40X): 1.8 mm x 100X/40X = Low power (100X): FOV diameter 1.8 mm = 1,800 um High power (400X): 1.8 mm x 100X/400X = 0.45 mm = 450 um Oil immersion (1000X): O.18m 180 um 1.8 mm x 100x/1000X = Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification 400x yoox FOV diam. FOV diam. um uni length length um
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