Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Question
Chapter 3, Problem 2A
Summary Introduction
To write:
The use of a good compound light microscope with a resolving power of 0.3 mm, a 10x and a 100x oil immersion lens.
Given:
“Compound light microscope” and “resolving power”
Introduction:
A compound microscope is consists of two convex lenses with shorter focal length, used to magnify the tiny objects. The resolving power of the compound microscope is 0.2µm and magnification power is 2000x.
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Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X oil immersion lens. Would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm?
Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.
Calculate the diameter of the field of view for each total magnification on your microscope in millimeters
(mm) and then convert this value to micrometers (um):
4.5 mm =
4,500 um
Scanning (40X):
1.8 mm x 100X/40X =
Low power (100X):
FOV diameter 1.8 mm =
1,800 um
High power (400X):
1.8 mm x 100X/400X = 0.45 mm =
450 um
Oil immersion (1000X):
O.18m
180 um
1.8 mm x 100x/1000X =
Draw and estimate the length of a single Euglena (high power) and Paramecium (low power):
Paramecium
Euglena
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Chapter 3 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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Similar questions
- Calculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4,500 um Scanning (40X): 1.8 mm x 100X/40X = 5 mm = %3D Low power (100X): FOV diameter = 1.8 mm = 1,800 um 1.8 mm x 100X/400X = 0.45 mm =450 um 180 um High power (400X): 1.8 mm x 100X/1000x = 28 mm = Oil immersion (1000X): %3D Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification FOV diam. um FOV diam. um length um length umarrow_forwardIn confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.arrow_forwardWhat is the final magnification that you would obtain if you were viewing an object through a microscope with a 40X objective lens and a 25.5X ocular lens?arrow_forward
- If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being used for observation, what is the total magnification for the viewed image? 10x 43x 1000x 400xarrow_forwardThe field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?arrow_forwardIf the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x, what is the magnification of the objective lens that was used? Paragraph BIUarrow_forward
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- The total magnification of a microscope is calculated by:(a) Addition of the objective lens and ocular lens magnifi-cation powers(b) Multiplication of the objective lens and ocular lens mag-nification powers(c) Multiplication of the objective lens and condenser lensmagnification powers(d) The objective lens power squared(e) None of the abovearrow_forwardOn a particular microscope, the numerical apertures of the condenser and the high dry power objective lenses are 1.25 and 0.65 respectively. You are supplied with a filter that selects a wavelength of 500 nm. A) What is the limit of resolution on this microscope when using the high dry power lens? (show your calculations) B) Will you be able to distinguish two points that are 260 nm apart as being separate or will they blur into one? (explain your answer)arrow_forwardFor a microscope that has an objective lens magnification of 100X and an ocular lens magnification of 5X, calculate the total magnification. 500X O 1000X O 50X O 100X O No listed answer is correct.arrow_forward
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