Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
Question
Book Icon
Chapter 3, Problem 4P
Interpretation Introduction

Interpretation:

The absorbance of a 1 mg/mL solution across a 1-cm path should be determined along with the percentage of transmission of incident-light.

Concept introduction:

The absorbance(A) of a solution is represented as

A=log10(I0/I)

In this equation I0 is the incident-light intensity.

Transmitted-light intensity is denoted by I.

The relation between extinction coefficient, concentration and path length is denoted as l (in cm) as:

A = εlc

Blurred answer
Students have asked these similar questions
Thank you for helping me. I have to questions. In solution B, if we choose two other points on the graph for calculating the slope, wouldnt the values for Km/Vmax be different than 6.1? Why did you choose exactly those points on the graph?  Another thing, I understand the method you used to get to Km/Vmax= slope, but how did you then get the value for Vmax to be 10 mM/s??
Substituent effects. What is the pHPpH of a 0.1 M0.1 M solution of chloroacetic acid (CICH2 COOH, pKa=2.86)? (CICH, COOH, pK, = 2.86) ? do
Help me fast with detail explanation. Definitely I will give Upvote.
Knowledge Booster
Background pattern image
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Biochemistry
Biochemistry
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:W. H. Freeman
Text book image
Lehninger Principles of Biochemistry
Biochemistry
ISBN:9781464126116
Author:David L. Nelson, Michael M. Cox
Publisher:W. H. Freeman
Text book image
Fundamentals of Biochemistry: Life at the Molecul...
Biochemistry
ISBN:9781118918401
Author:Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:WILEY
Text book image
Biochemistry
Biochemistry
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Cengage Learning
Text book image
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Text book image
Fundamentals of General, Organic, and Biological ...
Biochemistry
ISBN:9780134015187
Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:PEARSON